Glycosphingolipids (GSLs) are cell membrane lipids composed of sugars and lipid moieties that are important for cellular activity and physiopathological processes. CD BioGlyco provides a variety of different high-performance liquid chromatography (HPLC) services for GSL composition analysis based on different separation mechanisms.

How Does HPLC Work?

HPLC is a modern separation technique based on the mass transfer principle that uses a liquid as the mobile phase to partition the separated compounds alternately between the adsorbent and the liquid phase. HPLC separates and purifies the target compounds in the mixture by different processes such as adsorption, partitioning, reaction, and diffusion, and determines the presence and concentration of the targets by detection. Our HPLC-based compositional analysis services handle a variety of sample types, including gases, liquids, and solids, and our experts separate and characterize compounds to determine chemical structures quickly and accurately and provide customers with purified GSLs.

Our Service

HPLC has become one of the most popular and promising modern separation and analysis techniques and is used in many fields such as pharmaceuticals, food, environmental protection, cosmetics, etc. Based on different column structures and reaction mechanisms, CD BioGlyco offers the following component analysis services for GSLs:

• GSL component analysis services based on RP-HPLC (reverse-phase high-performance liquid chromatography) This service is suitable for the separation of non-polar compounds and is also used for the separation, purification, and quantification of other biological macromolecules not limited to GSL, such as proteins, nucleic acids, glycans, etc.
• GSL component analysis services based on NP-HPLC (normal phase high-performance liquid chromatography)
• GSL component analysis services based on IEC-HPLC (ion exchange chromatography-high performance liquid chromatography

This service is suitable for complex samples with different molecular weights, charges, and polarities, especially for non-volatile ionic compounds.

• GSL component analysis services based on GC-HPLC (gas chromatography-high performance liquid chromatography)

This service combines the separation mechanisms and respective advantages of gas chromatography and HPLC to improve the selectivity, sensitivity, and resolution of separation analysis.

Fig1. The HPLC technologies at CD BioGlyco. (CD BioGlyco)

Applications

• Active ingredients in nutrition and health products
• Study of biosynthesis and biological transformation of GSLs

Advantages of Us

• High accuracy and high separation capacity
• High repeatability
• One-stop service for isolation, purification, and structure identification

CD BioGlyco provides clients with an industry-leading geochemical research laboratory and reliable HPLC-based GSL component analysis service. Our research team hopes that our extensive research experience in specialized areas will help you accelerate the progress of your research. If you are interested in our services, please contact us to obtain more information.

Reference

1. Corradini, D.; et al. Handbook of HPLC. CRC Press, 1998.