Glycosphingolipids (GSLs) are composed of carbohydrate and lipid moieties, mainly found on the extracellular surface, and their main role is to participate in biological functions such as cell signaling, cell recognition adhesion, and pathogen recognition. CD BioGlyco is committed to providing specialized component analysis services by thin-layer chromatography (TLC) for GSLs.
TLC is a technique used for the separation and identification of constituents in various biological samples based on the separation of a component from a mixture by adsorption of a stationary phase. TLC plates coated with stationary phase are used to separate the target compound from the mixture based on its physical and chemical properties. The various components present in a sample can be quantified and identified based on the position of the products on the plate and the phenomenon of their reaction with specific reagents. This technique is useful for the analysis of a wide variety of biological samples, including tissue extracts and biological fluids, and provides valuable information about the composition and structure of these samples.
Fig.1 Separation of black ink on a TLC plate. (Wikipedia)
The most commonly used stationary phases for TLC are adsorbents, such as silica gel, alumina, diatomaceous earth, fine powder of cellulose and polyamide, etc. Depending on the type of sample and assay, CD BioGlyco offers various TLC-based GSL composition analysis services including:
Our service offers a wide range of affinity and selectivity, thus allowing for highly targeted purification of samples.
Polyamide chromatography can be used to separate various biological macromolecules, including proteins, nucleic acids, peptides, etc. Depending on the ligand and method, it also is used for chemical separations and extractions.
We use micron-sized cellulose particles as a stationary phase to separate and purify biological macromolecules, such as proteins and nucleic acids, under weakly acidic conditions.
This service allows the separation and detection of most organic compounds including substances with different polarities, such as aliphatic compounds, aromatic compounds, and polar compounds.
Fig.2 The analysis technologies at CD BioGlyco. (CD BioGlyco)
CD BioGlyco has a well-established component analysis laboratory and experienced researchers to provide optimized GSL component analysis to our customers. Our dedicated research team is proud to be able to provide different types of analytical services according to the specific needs of our clients. If you are interested in our services, please contact us to obtain more information.
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