Properties
Description
Hexokinase from yeast can be used for catalyzing the initial procedure of glycolysis which converts glucose and ATP to glucose-6-phosphate. Glucose-6-phosphate dehydrogenase from yeast is a considerable enzyme in the pentose phosphate pathway that leads to the constitution of 6-phosphogluconolactone and NADPH to glucose-6-phosphate and NADP+.
Unit Definition
Hexokinase: One unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
Glucose-6-phosphate Dehydrogenase: One unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.
Applications
Hexokinase/Glucose-6-phosphate dehydrogenase from yeast applies to the detection of some hexoses in carbohydrate studies and the dairy industries.
