High-performance liquid chromatography (HPLC) has been widely used as a sensitive technique for monitoring protein glycosylation. CD BioGlyco is committed to providing a wide range of glycan profiling services through HPLC methods for customers.
HPLC, as a technique for the separation and analysis of glycoproteins and their derived carbohydrates, has gained significant interest in recent years. HPLC is an important branch of chromatography. With liquid as the mobile phase and high-pressure infusion system, the mobile phases such as single solvent with different polarity, mixed solvents with different proportions, and buffer are pumped into the chromatographic column equipped with the stationary phase. After being separated in the column, components enter the detector for detection to realize the analysis of samples.
According to the characteristics of glycans and the HPLC matrix used, the released glycans can be separated into multiple components. Normal phase and reverse phase chromatography are often used in combination with fluorescent-labeled oligosaccharides to separate sialylated and neutral N- and O-linked glycans. In addition, a method of high pH anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) has been successfully used to separate and quantify the constituent monosaccharides released by acidic hydrolysis of glycan chains and to separate N-linked oligosaccharides separated by enzymatic digestion.
Fig.1 Separation of desialylated N-glycans from α1-acid glycoprotein using HPAEC-PAD. Peaks1 and 2 are internal standards used for calibration of the chromatographic system. (Gohlke, 2019)
CD BioGlyco has successfully developed a variety of advanced and sensitive strategies for glycoprotein analysis based on HPLC technology. The technologies we provide include but are not limited to:
Finally, we will provide detailed reports including experimental procedures, liquid chromatography, and mass spectrometry parameters, glycan profiling results, and bioinformatics analysis for customers.
As a leading biotechnology company in the field of glycobiology, researchers at CD BioGlyco constantly seek new and feasible strategies to analyze glycans on glycoproteins to provide customers with structural analysis services of glycans. We have cooperated with numerous scientific research institutions and companies and accumulated rich successful experience. Please contact us for further assistance if you have any questions.
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