Description of HPLC

With the development of biotechnology, certain important biological preparations and pharmaceuticals, which are mainly composed of biological macromolecules, often fail to meet the required purification requirements by using traditional separation and purification techniques (ultrafiltration, precipitation, extraction, and centrifugation, etc.). Therefore, the focus on separation and purification techniques has been shifted to chromatography. In recent years, high-performance liquid chromatography (HPLC) has been at the forefront of contemporary high-performance separation and purification techniques. HPLC is a very important separation and purification technique in organic chemistry and biochemistry. It is divided into normal-phase chromatography and reversed-phase chromatography. Normal-phase chromatography and reversed-phase chromatography can be further divided into adsorption chromatography and polar chemical bonding chromatography. Since polar compounds are more easily retained by polar stationary phases, normal-phase chromatography is generally more suitable for the separation of polar compounds, and reversed-phase chromatography is generally suitable for the separation of non-polar and weakly polar compounds.

Fig.1 DNA and RNA structure. (Wikipedia)

HPLC-based DNA and RNA Purification Service at CD BioGlyco

DNA and RNA are two biomolecules that require high purity for various applications. At CD BioGlyco, we offer HPLC technology for the separation and purification of DNA and RNA to produce higher-purity DNA and RNA. The HPLC process utilizes the "adsorption-elution" principle, and we offer different separation modes for different substances, as shown in Table 1.

Table 1 Sample selection for separation.

In addition to this, the packing of the column has different effects on the separation of substances, and we offer the main packing materials:

• Al₂O₃ matrix: stable to strong acids and bases;
• Polymer matrix: stable to strong acid and alkali, surface chemistry can be carried out according to the needs of different chromatography;
• Silica gel matrix: high mechanical strength, fast separation speed, and good reproducibility, required for strong alkali separation.

Fig.2 Process of sample isolation and purification. (CD BioGlyco)

Applications

• HPLC could be used for the separation and purification of molecules of different molecular weights.
• HPLC is used for the separation and purification of pharmaceutical intermediates.
• HPLC could be used to assist in the development of DNA and gene technology.

Advantages

• HPLC is an excellent method of separation and purification and hence it helps in studying the structure of DNA and RNA.
• HPLC technology helps to study DNA technology and gene technology in depth.

CD BioGlyco is a company with extensive experience in DNA and RNA research. We have professional research equipment for the efficient isolation and purification of DNA and RNA for our clients, and our high-quality service has earned us a good reputation around the world.

Clients can contact our staff directly and we will reply promptly. If you are interested in our services, please feel free to contact us for more details.