Application of 96-Well Plate Platform in Glycan Analysis

A 96-well plate platform is used to process multiple samples or reactions simultaneously. It is usually made of plastic or glass and has a flat bottom and 96 wells, each of which can hold one sample. 96-well plate platforms are widely used for high-throughput (HT) experiments such as enzyme activity assays and polymerase chain reactions (PCR). By adding different reagents, samples, or reaction systems to different wells, large-scale experimental operations can be performed quickly and in parallel. This platform is designed to help increase experimental efficiency, save time and resources, and automate the processing and reading of data. Combining the platform with analytical instruments such as a microplate reader, high-performance liquid chromatography (HPLC), or mass spectrometry (MS), the detection instrument HT generates corresponding data for each small well to provide information on individual sample molecules. This HT sample analysis method has been applied in biomedical research, proteomics, and drug development.

Fig.1 Strategy for glycan release, fluorescent labeling, NPHPLC profiling, structure assignments, and relative quantitation. (Royle, et al., 2008)

High-Throughput Glycan Screening Service Using a 96-Well Plate Platform at CD BioGlyco

CD BioGlyco provides clients with specialized High-Throughput Glycan Screening (HTS) Services based on a 96-well plate platform. The services we provide include but are not limited to:

• HT analysis of structural changes in the glycan chain
  We perform large-scale glycan chain structure analysis by adding glycanase treatment products or hydrolysis products from different samples to different small wells of a 96-well plate. The composition and structure of different glycan chain samples are evaluated by MS and other techniques to help you detect changes in glycan chain modifications.
• HTS of glycan chains for biological activity and function
  We mimic the interactions that occur between glycans and organisms by immobilizing glycans of different structures or origins at the bottom of the wells and adding samples with specific biomarkers or receptors. Subsequently, glycan chains that are biologically active and functional for specific biological processes or biomarkers are screened by washing and analyzing steps.
• HTS of fluorescent probes for glycans
  Different glycan samples are immobilized in each well and then probes with fluorescent properties are added. Subsequently, non-specific binding substances are removed by a washing step. Finally, the interaction between the different glycans and the fluorescent probes is evaluated by measuring the fluorescence intensity or image in each well using a device such as a fluorescence reader.

Fig.2 HTS of glycans based on the 96-well plate platform. (CD BioGlyco)

Applications

• Pathogen detection
• Conversion to biofuels through fermentation or other methods
• Improved crop storage properties

Advantages of Us

• High efficiency
• Customized experimental details
• Exceptional service in biotech

CD BioGlyco is committed to providing our clients with the highest-quality HT glycan screening using a 96-well plate platform, utilizing the latest technology and innovative solutions to help our clients advance their research. If you are interested in our services, please feel free to contact us for more details.