Application of ESI-MSn in Glycan Analysis

Electrospray ionization and multi-stage mass spectrometry (ESI-MSn) combine the analytical techniques of ESI and MSn. ESI-MSn is widely used in the field of bioanalysis and especially plays an important role in the analysis of the structure and composition of biomolecules, such as glycans, proteins, and nucleic acids. ESI is a type of ionization technique, which is used to convert molecules in solution into charged ions by spraying them with an ESI ion source under a high voltage to convert molecules in solution into charged ions. The ESI ion source uses a needle or capillary to eject high-speed droplets to form a microscopic electrospray in which molecules in solution are ionized and charged. MSn refers to multistage mass spectrometry (MS), which involves acquiring MS data from complex samples in a mass spectrometer by continuously performing multiple tandem MS scans. ESI-MSn combines an ESI ion source and multistage MS to provide more detailed and comprehensive mass spectral data.

ESI-MSn-based High-Throughput Glycan Screening Service at CD BioGlyco

ESI-MSn enables high-throughput glycan screening and rapid analysis of glycan structures and modifications in a large number of samples. This is extremely useful for glycomics research, metabolomics, and drug screening of glycans in drug discovery and development. CD BioGlyco offers ESI-MSn-based high throughput glycans screening service to our clients, our services are as follows:

• High-throughput glycan modification analysis
  Our professional team helps you detect and quantify modifications in glycans, such as acetylation, methylation, phosphorylation, etc., and reveal their structures and connections. The location and type of modifications are determined by measuring the mass and abundance of fragment ions.
• High-throughput glycan structure analysis
  We provide high-throughput structural analysis services for glycans. By analyzing the mass and abundance of fragment ions, we identify specific structural information of glycans for you, including branching positions, ring structures, stereo conformations, and more.

Fig.1 ESI-MSn-based high-throughput glycan analysis process. (CD BioGlyco)

Applications

• Drug development and efficacy assessment
• Glycan structure identification and glycomics studies
• An in-depth look at the interrelationship between glycans and disease

Advantages of Us

• No special markings required
• Large-scale sample screening and quantitative analysis
• Client-centric approach

CD BioGlyco provides clients with high-throughput glycan screening services based on ESI-MSn technology by using advanced MS analytical techniques. We always adhere to strict quality control standards and work closely with our clients to ensure that their specific needs are met. If you need a long-term partner in the field of glycobiology, please feel free to contact us and we will be delighted to help you in your related research.