Microchip-Based Assay Used for High-Throughput Glycan Screening

Microchip technology is an HTS method that can be used to detect and analyze a large number of biomolecules, including proteins, nucleic acids, and glycans. In the case of glycan analysis, microchip technology detects the interaction between glycans and specific molecules through a variety of chemical reactions. Specifically, a series of molecules, such as antibodies, metal ions, and fluorescent probes, are first pre-immobilized in the microchip, which can specifically bind to different parts of the glycan molecule. Then, the glycan molecules in the sample to be examined will react with the molecules immobilized on the chip to form a chemical complex. These complexes can be quantitatively analyzed and imaged immediately thereafter using laser scanning or other imaging techniques. By analyzing the changes in the number and geometric position of the chemical complexes in different samples, information such as the type and content of glycans in each sample can be determined.

Fig.1 Flow chart of HTS of glycans by microchip-based assay. (CD BioGlyco)

High-Throughput Glycan Screening Using Microchip-Based Assay at CD BioGlyco

CD BioGlyco specializes in the development and provision of microchip-based high-throughput glycan screening services. After careful design and optimized experimental protocols, we help clients obtain glycan high-throughput screening (HTS) results in a very short period, greatly improving the efficiency and quality of work. Our services encompass a wide range of offerings, including but not restricted to:

• Glycosyl group specificity HTS service
  We take advantage of the feature that the microchip can be coated with different glycan groups, such as mannose, galactose, glucose, etc., thus providing HTS of specific glycan groups.
• Glycans HTS service with specific probes
  We immobilize oligonucleotides or proteins with specificity, such as arachidonic acid binding proteins, on microchips to screen for glycans that bind specifically to these probes for our clients.
• High throughput glycan immunoassay service
  This method allows for the immobilization of different glycan antibodies on microchips and immune reactions under specific conditions, thus screening the glycans that bind to the antibodies for the clients.

Applications

• Vaccine development
• Research on glycosylated drugs
• Glycemic biomarker research

Advantages of Us

• High analytical sensitivity and repeatability
• The large volume of data
• High versatility and wide range of adaptations

CD BioGlyco combines advanced microfluidics and glycosylation methods with highly sensitive microchip technology to provide clients with microchip-based high-throughput glycan screening services. Our services can be widely used in the life science, pharmaceutical, and chemical industries to provide high-quality glycan screening solutions to our clients. If you are interested in our services, please contact us for further information and inquiries.