Human Fibrinogen Evaluation Service
Promote Your Project Development with High-quality Human Fibrinogen Evaluation
CD BioGlyco has sophisticated Pharmaceutical and Biological Analysis systems for analyzing and evaluating different samples for our clients all over the world such as Pharmaceutical Analysis, Biological Evaluation, and Pharmaceutical Excipient Analysis. Human fibrinogen is a protein that is synthesized mainly by hepatocytes and has a clotting function. We rely on a team of professionals to provide comprehensive evaluation services for human fibrinogen and human fibrin sealant kits. Moreover, we provide high-quality human immunoglobulin evaluation and human thrombin evaluation services.
- Basic analysis
Our experienced operators provide the following specialized analytical services:
- pH test
- Vacuum test
- Moisture measurement
- Purity test
- Reconstitution time test
- Visible particulate test
- Coagulation assay
- Osmolatity test
- Structural analysis
- We analyze the individual crystal structures of human fibrinogen by X-ray diffraction. Moreover, we provide amino terminus sequencing analysis of the crystals after X-ray diffraction.
- Quantitative analysis
- Total fibrinogen: Our team uses the Kjeldahl method to determine the total fibrinogen content in human fibrinogen.
- Citrate ion: Our team provides accurate citrate ion content analysis service by colorimetric method or high-performance liquid chromatography (HPLC).
- Glucose or sucrose: Cation exchange chromatography is used to determine the sugar content of human fibrinogen, such as glucose or sucrose.
- Chlorine ion: Use ammonium thiocyanate titration to determine the chloride ion content of the samples.
- Amino acid: We use a C18 reversed-phase column for the derivatization of the sample.
- Tributyl phosphate residue: Our researchers use gas chromatography (GC) to determine tributyl phosphate residues in human fibrinogen.
- Polysorbate 80 residue: Our team rapidly determines polysorbate 80 residue in human fibrinogen by colorimetry.
- Impurity protein analysis
- Our team identifies and screens impurity proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), mass spectrometry (MS), or enzyme-linked immunosorbent assay (ELISA).
- Bioactivity analysis
- We provide professional abnormal toxicity tests, pyrogen tests, sterility tests, and HBsAg test services.
![Schematic diagram of human fibrinogen evaluation tools. (CD BioGlyco)](https://www.bioglyco.com/wp-content/themes/glycobio/img/4-14-2-11-human-fibrinogen-evaluation-service-1.jpg)
Publication Data
Technology: MS, SDS-PAGE, HPLC
Journal: Nature Communications
Published: 2020
IF: 17.694
Results: In this work, researchers used differential cysteine alkylation and MS to determine the disulfide state of fibrinogen in human hepatocytes. Multiple analyses showed that fibrinogen is produced in multiple disulfide-bonded or covalent states and functions in multiple covalent forms. Further analyses revealed that disulfides are formed by the polymerization of fibrin and play a key role in the functioning of the protein. Notably, the polypeptide backbone of fibrinogen does not exist in a single covalent form in circulation but may be in hundreds of different covalent forms.
Fig.1 Human fibrinogen analysis profile. (Butera & Hogg, 2020)
Applications of Human Fibrinogen Evaluation
- Human fibrinogen evaluation has broad implications for drug resistance and drug development such as fibrinogen receptor antagonists and fibrinolytic agents.
- Human fibrinogen evaluation provides new analysis routes for studying the protein composition of blood products.
Advantages of Us
- Our talented team has not only a wealth of expertise but also excellent human fibrinogen analytical skills to ensure that complex problems are solved quickly.
- We offer specialized structural and analytical and quantitative analysis services to meet the diverse needs of our clients.
- Our professional analyst team quickly delivers project analysis reports, raw data, analysis conditions, etc.
CD BioGlyco has an efficient analysis and evaluation team to provide comprehensive pharmaceutical and biological analysis services to our clients. No matter what problems you encounter in your biological evaluation, please feel free to contact us.
Reference
- Butera, D.; Hogg, P.J. Fibrinogen function achieved through multiple covalent states. Nature Communications. 2020, 11(1): 5468.
This service is for Research Use Only, not intended for any clinical use.