Properties
Description
Human lipopolysaccharide-Ab (LPS-Ab) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of human LPS-Ab as low as 1 EU/L and its detection range is 25-800 EU/L.
Assay Principle
Human lipopolysaccharide-Ab (LPS-Ab) ELISA kit is specifically designed for measuring the level of human LPS-Ab in a given sample. To perform the assay, purified human LPS-Ab antibodies are used to coat the wells of a microtiter plate, creating a solid-phase antibody. Next, the LPS sample is added to the wells, resulting in the binding of LPS molecules to the antibodies. The antibodies are labeled with HRP, forming an antibody-antigen-enzyme-antibody complex. After thorough washing, a TMB substrate solution is added to the wells, causing the TMB substrate to undergo an HRP enzyme-catalyzed reaction, leading to a color change to blue. To terminate the reaction, a sulphuric acid solution is added, and the resulting color change is measured using a spectrophotometer at a wavelength of 450 nm. Finally, the concentration of Porcine LPS in the samples can be determined by comparing the optical density (O.D.) of the samples to a standard curve.
ELISA Type
Sandwich ELISA kit
Assay Type
Sandwich ELISA kit
Components
Closure plate membrane; Sealed bags; Micro ELISA stripplate; Standard; HRP-Conjugate reagent; Sample diluent; Chromogen solution A; Chromogen solution B; Stop solution; Wash solution
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Human lipopolysaccharide-Ab (LPS-Ab) ELISA kit can be used to measure the level of human LPS-Ab in serum, plasma, tissue homogenate and other biological fluid samples.
