Description
Exo-α-sialidase from Clostridium perfringens is responsible for hydrolyzing terminal N- or O-acetylneuraminic acids which are α2,3-, α2,6-, α2,8- linked to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids.
Unit Definition
One unit of sialidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-α-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 37°C.
Applications
Exo-α-sialidase from Clostridium perfringens can be used in clinical chemistry, glycobiology, and carbohydrate research.
