CD BioGlyco has professional glycomedicine development teams with multiple world-leading technologies to help customers comprehensively promote the development of carbohydrate-based glycomedicine. Our advanced technology platforms and high-quality services will fully meet your needs. We have the confidence to be your essential research assistant in the field of glycobiology.

In Vitro Anticancer Activity Screening Techniques

Currently, chemotherapy, surgery, radiotherapy, biotherapy, and other adjuvant therapy are common treatments for tumors. However, chemotherapy is the preferred and the most common treatment for patients in clinical practice. Yet, it still lacks effective antitumor drugs, while some patients easily acquire resistance to many new drugs after a period of treatment. Cancer cells are well known to display an enhanced sugar uptake and consumption. An increasing number of researchers from all over the world have been focusing on carbohydrate-based glycomedicine to find new antitumor drugs.

The MTT/MTS in vitro cell proliferation assay is one of the most widely used assays for evaluating the preliminary anticancer activity of glycomedicine. The highly reliable, colorimetric-based assay is readily performed on a wide range of cell lines. These are two of the most useful anticancer screening techniques available that are relatively economical and can be easily and routinely performed in the laboratory to characterize anticancer activity. Kinase inhibition assays are also one of the most widespread enzyme inhibition screening assays performed. Kinases are enzymes that play a key role in a number of physiological processes and their inhibitors have been found to exhibit anticancer activity against various human cancer cell lines.

Fig.1 Different types of in vitro tumor models. (Kitaeva, et al., 2020)

In Vitro Antitumor Activity Screening Services

CD BioGlyco provides high-quality in vitro screening services for the inhibitory effect of glycomedicine on tumor cell proliferation. Our services include but are not limited to:

• Determination of the effect of glycomedicine on tumor cell apoptosis by the MTT/MTS assay and flow cytometry.
• Kinase inhibition assays.
• To explore the effects of glycomedicine on the cell cycle progression of cancer cells.
• Expose cancer cells to drugs and examine the expression of selected proteins in cells by western blot.

Advantages of Us

• Professional antitumor activity screening expert team.
• Experienced and well-trained technicians.
• Advanced instruments and equipment.
• Optimal experimental conditions.

CD BioGlyco is committed to ensuring that every service provides the highest level of satisfaction. Our first-class experimental platform and rich experience ensure customers with high-quality customized in vitro antitumor activity screening services. If you are interested in our services, please contact us to obtain more information.

Reference:

1. Kitaeva, K.V.; et al. Cell culture based in vitro test systems for anticancer drug screening. Frontiers in Bioengineering and Biotechnology, 2020, 8: 322.