Inert Alkyl Chain-based Cell Surface Metabolic Oligosaccharide Engineering Service

What Is Metabolic Oligosaccharide Engineering (MOE)?

Cell surface engineering (i.e., the precise regulation of cell surface components by physical, chemical, and biological means) is an important window to study the basic behaviors of cell metabolism, differentiation, and communication, and has promising applications in the development of next-generation cellular imaging technologies and cell therapies. MOE skillfully utilizes the cellular metabolic glycan pathway to assemble glycan units with special chemical groups into the glycocalyx on the surface of the cell membrane, thus presenting reactive chemical groups on the cell membrane. Then, through bioorthogonal reactions, synthesized polymers and nanomaterials can be mounted onto the cell surface. In this way, the metabolic fate of monosaccharides in the cell can be understood. Bioorthogonal targeting achieved by combining metabolic oligosaccharide engineering and click chemistry has the potential to selectively bind therapeutic agents to a range of specific tissues.

Fig.1 Metabolic oligosaccharide engineering with unnatural derivatives of glycan allows "hijacking" of biosynthetic pathways of endogenous glycan analogs to install biorthogonal handles (R₁) for chemoselective ligation. R₂ = OH or Ac. (Tomás & Gibson, 2020)

Inert Alkyl Chain-based Cell Surface MOE Service at CD BioGlyco

At CD BioGlyco, we have a permanent commitment to the development of Cell Surface Glycoengineering techniques. We provide Cell Surface Metabolic Oligosaccharide Engineering Services to our clients.

• Cell surface metabolism inert alkyl chain oligosaccharide function exploration
  We graft inert groups onto cell surface oligosaccharide chains to obtain inert alkyl chain oligosaccharides. The obtained oligosaccharide containing inert alkyl groups is both hydrophobic and oleophobic, with low surface energy, which makes it easier to be adsorbed on the surface of the cell membrane and smoothly pass through the cell membrane as well as biological barriers such as the membrane of endosomes.
• Cell surface modification
  We deliver unique reactive ketone groups to endogenous cell surface sialic acid residues by treating cells with ketone-treated cells, where ketones react with complementary nucleophilic reagents (e.g., amyloxy and hydrazide groups) in highly selective condensation reactions to lay the groundwork for modifying cell surfaces with novel epitopes at defined cell surface densities. We also determine the binding properties of the remodeled cells by flow cytometry analysis.

Fig.2 Technologies for inert alkyl chain-based cell surface MOE. (CD BioGlyco)

Applications

• Cell surface metabolic oligosaccharide engineering can be utilized in cell engineering fields such as biomolecule capture, microfabrication, drug delivery, and immunomodulation.
• Cell surface metabolic oligosaccharide engineering based on inert alkyl chains can be used to study carbohydrate-mediated cell surface interactions.
• Cell surface metabolism oligosaccharides based on inert alkyl chains can be used to prepare materials for use in biotechnology such as protein transfection.

Advantages of Us

• Metabolic oligosaccharide engineering allows the synthesis of cell surface oligosaccharides with different structures.
• Our cell surface oligosaccharide engineering helps researchers explore the diverse biological functions of cell surfaces.
• Cell surface oligosaccharide engineering helps to study the structure of oligosaccharides and their roles in various biological processes, including cell signaling and immune responses.

CD BioGlyco provides comprehensive glycoengineering techniques to our clients. Our technicians are reliable and efficient. At the same time, every step of the services we offer has been subjected to several quality tests to ensure client satisfaction. If you are interested in our services, please feel free to contact us.