The structural analysis methods of glycans have been lagging behind nucleic acids, proteins, and lipids, which limits the development of disciplines related to glycochemistry. CD BioGlyco has advanced experimental instruments to analyze and identify the structure of carbohydrates. We look forward to being your indispensable research assistant in the field of glycobiology.

Background

Due to the obvious diversity of glycans structure and expression in nature, there are still many difficulties in the analysis of glycans structure, and little is known about the details of this diversity. The difficulty of glycan analysis lies not only in its variety but also in its complex structure. In fact, many naturally occurring glycans of prokaryotes and eukaryotes have few similarities, and organisms in each category also have obvious diversity in glycans.

The technology of mass spectrometry is considered to be an important and indispensable means in various methods of structural analysis of polysaccharides. Fast atom bombardment, matrix-assisted laser desorption ionization, electrospray ionization, tandem mass spectrometry, and other mass spectrometry technology have been successfully applied to the structural analysis of polysaccharides. The structure of glycans is closely related to their functions, and the research on the structure of glycans directly affects the development of glycopharmacology. Therefore, the structure analysis of glycans is of great significance.

Fig.1 Glycan-isoforms of transferrin in cerebrospinal fluid. (Hoshi, 2017)

Services

CD BioGlyco analyzes the isoform structure of glycans by mass spectrometry (MS) technology, which can not only provide a variety of reliable sample structure information and high detection sensitivity but also have the ability of quick qualitative analysis. Services we provide include but are not limited to:

• Analysis of glycans isoform structures by MS-based technology, including electron impact mass spectrometry (EI-MS), fast atom bombardment mass spectrometry (FAB-MS), electrospray ionization mass spectrometry(ESI-MS), matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), GC–MS and LC-MS.
• Distinguishing isoform of glycans with high sensitivity and high resolution, using ion mobility spectrometry (IMS).

Applications

• Research on macro/microheterogeneity of glycans
• Research on the biological function of glycans
• Research on biomarkers for cancer diagnosis

Advantages of Us

• Various instrument types
• Outstanding and professional teams
• Formulation of characteristic detection schemes

CD BioGlyco has high-tech analysis equipment and excellent teams to provide perfect one-stop services for clients. We have supplied professional technical services to thousands of enterprise customers and accumulated lots of successful experiences. Please feel free to contact us if you need any further information.

References:

1. Čmelík, R.; Chmelík, J. Structural analysis and differentiation of reducing and nonreducing neutral model starch oligosaccharides by negative-ion electrospray ionization ion-trap mass spectrometry. International Journal of Mass Spectrometry. 2010, 291(1-2): 33-40.
2. Hoshi, K.; et al. A unique glycan-isoform of transferrin in cerebrospinal fluid: a potential diagnostic marker for neurological diseases. Biochimica et Biophysica Acta (BBA)-General Subjects. 2017, 1861(10):2473-8.