What Is Isomaltose?

Isomaltose is a disaccharide derived from maltose, which is formed by connecting two glucose monomers through α1,6 glycosidic bonds. It is a dimer of glucose and can be used as a substitute for sucrose which is suitable for diabetic patients and people prone to diabetes. Isomaltose exists in a variety of foods in nature, containing wheat, rice, corn, potatoes,etc., which can be hydrolyzed by the enzyme α-glucosidase in the human digestive system to generate glucose molecules. Therefore, isomaltose is a carbohydrate that can provide energy. Isomaltose has a sweet taste and is often used as a food additive and sweetener. It has anti-crystallization and high-temperature resistance, which improves the texture and maintain the stability of products.

Fig.1 The structure of isomaltose. (Wikipedia)

Isomaltose Analysis Services at CD BioGlyco

• High-performance Liquid Chromatography (HPLC)

The method using the amino column as the separation column, acetonitrile as the mobile phase, and differential refraction for detection has become the industry standard for quality control of isomaltooligosaccharides, which is suitable for the determination of isomaltose content in a complex matrix.

• Ion Chromatography-pulse Amperometric Detection (IC-PAD)

The method is suitable for the determination of isomaltooligosaccharides in various foods, with strong method specificity and high accuracy of results.

• Thin-layer Chromatography (TLC)

The method is used to determine the content of isomaltose, and the required equipment is simple and easy to operate.

• Capillary Electrophoresis (CE)

CE detects active ingredients in isomaltooligosaccharide products, it has the advantages of rapidity, high efficiency, and low operating costs.

• Gas Chromatography (GC)

Since sugars are non-volatile substances, sugars are derivatized into volatile substances before analysis by this method.

• Supercritical Fluid Chromatography (SFC)

Supercritical fluid is used as the chromatographic mobile phase, and various types of longer chromatographic columns or capillary columns are used as separation columns to analyze sugars at lower temperatures.

Fig.2 Multiple analytical methods of isomaltose. (CD BioGlyco)

Publication

Paper Title: Quantification of sugars in wheat flours with an HPAEC-PAD method

Technology: HPAEC-PAD

Journal: Food Chemistry

IF: 9.231

Published: 2015

Results: The author developed and validated an HPAEC-PAD method for quantifying various sugars (including isomaltose) in wheat flour. The authors optimized the conditions for sample preparation and finally prepared the sample of wheat flour in advance through water solid-liquid extraction and Carrez II protein precipitation. Hamilton RCX- 30 was used as the chromatographic column and the gradient solvent system was used as the mobile phase to carry out quantitative determination of various sugars. The method has been verified to have good recovery and repeatability, and various sugars were separated. Currently, the method has been successfully applied to the quantification of various sugars in wheat flour.

Fig.3 Sugar peaks after treating wheat flour with different extractants. (Pico, et al., 2015)

Applications

• Food industry: Isomaltose analysis ensures that the content and purity of isomaltose in food raw materials and products meet the specified standards or formula requirements, which is helpful for the control and optimization of food processing.
• Drug study and production: Detect and quantify the possible isomaltose residues in drug formulations to ensure the quality of drugs in the process of drug study and production.
• Biological sciences: In the field of biological science research, isomaltose analysis is used to evaluate and quantify the isomaltose content in cell culture media, culture products, or fermentation products, which is of great significance for regulating cell metabolism, optimizing culture conditions, and studying microbial fermentation process.

Advantages

• CD BioGlyco has advanced analytical equipment capable of accurately determining the isomaltose content in samples.
• We have established strict quality control measures and standardized operating procedures to ensure the high accuracy and reliability of isomaltose analysis results.
• We receive samples promptly, quickly analyze isomaltose, and provide analysis reports in a short time to meet clients' urgent needs.

CD BioGlyco has developed one-stop solutions for Carbohydrate Metabolism Analysis. We provide personalized isomaltose analysis services based on project requirements to meet clients' specific needs. If you need high-quality analysis services, we are your best choice. Please feel free to contact us promptly.

References:

1. Xu, J.J.; et al. Detection of functional isomaltose in food by ion chromatography-pulse amperometry. Food Industry Science and Technology. 2021, 42(13): 262-267.
2. Pico, J.; et al. Quantification of sugars in wheat flours with an HPAEC-PAD method. Food chemistry. 2015, 173, 674-681.