LNH plays an active role in the growth and development of infants, especially in the aspect of shaping the internal microbiota. CD BioGlyco supplies suitable synthesis methods to achieve efficient production of LNH.

Effect of LNH on the Microbial Community

Human milk oligosaccharides (HMOs) are a class of non-conjugated glycans with diverse structures and are the third most abundant solid component of human milk after lipids and lactose. HMO will not be digested by infants but will be used as a prebiotic for beneficial bacteria, thus maintaining the balance of intestinal microflora. In early infancy, the pioneer intestinal microbiota may be susceptible to antibiotics, while the prebiotic oligosaccharides in human milk and some infant formula may protect healthy infant intestinal microbiota. The latest research has proved that two kinds of non-fucosylated HMOs are associated with microbial communities: LNH and 6'-sialyllactose, which are respectively related to the higher and lower relative abundance of Bifidobacterium.

Fig.1 The structure of LNH. (Bandara, et al., 2019)

The Exploration Process on LNH

LNH was first isolated and characterized from human milk by the Kobata group in 1927. Later, LNH was also found in other mammals such as Dutch Landrace sows. A recent study found that low concentrations of fucose-L-lactose-N-hexose or LNH were associated with early cessation of breastfeeding. The reports on LNH also include many related derivatives, such as fucosylated LNH. They all have a complex relationship with the development of infant intestinal flora. In the future, it is necessary to conduct further research to determine how LNH affects infant health.

LNH Production Service at CD BioGlyco

CD BioGlyco has accumulated various Synthetic technologies including purification, chemical, enzymatic, chemoenzymatic, and metabolic engineering methods to produce LNH. The optimization strategies we supply include but are not limited to the following:

• Optimized and mature separation and purification technology
• Enzymatic synthesis
• One-pot enzymatic system.
• A pot of multiple enzymes includes bacterial glycosyltransferases and sugar-nucleotide–generating enzymes.
• Selection and identification of suitable enzymes
• Optimal enzyme expression levels.
• Microbial Fermentation
• Construct metabolically engineered strains.
• Optimization of strain culture conditions.
• Chemoenzymatic synthesis
• Combining chemical carbohydrate synthesis with selective enzymatic glycosylation strategy.
• Precursors are obtained by chemical synthesis, then the sequence of galactosyltransferase-catalyzed reactions is rationally arranged.

Advantages of Us

• One-step reaction synthesis.
• Response specificity.
• High productivity and efficiency.
• Combined application of production technologies.

CD BioGlyco has made many achievements in the field of production services of LNH, which are widely praised by clients internationally. If you have any further queries, please contact us directly for more details.

Reference

1. Bandara, M.D.; et al. Chemical synthesis of human milk oligosaccharides: lacto-N-hexaose Galβ1→ 3GlcNAcβ1→ 3 [Galβ1→ 4GlcNAcβ1→ 6] Galβ1→ 4Glc. The Journal of organic chemistry. 2019, 84(24): 16192-16198.