Lactose N-trisaccharide II (LNT II) is the core structural unit of human milk oligosaccharide which has many potential nutritional values. Based on years of experience in providing services in the field of glycobiology, CD BioGlyco provides professional and high-quality services for LNT II production.

Existence of LNT II in the Human Body

As an important skeleton, LNT II has a wide range of application prospects that can be further synthesized into different human milk oligosaccharides (HMOs) as raw materials, such as lacto-N-tetraose and lacto-N-neotetraose. LNT II is a core structural unit of HMOs and it has attracted substantial attention for its multiple potential functions for infants.

Fig.1 Sequential strategy for the synthesis of LNT II and LNnT. (Liu, et al., 2020)

LNT II is located on the cell surface as a glycolipid component normally. In the 1990s, there was a great interest in developing synthetic pathways to this type of oligosaccharide unit, because it usually occurs as a common trisaccharide unit in the lactotriglycosylceramide corresponding to the glycosphingolipids of the lactose series. The oligosaccharide components of glycosphingolipids have numerous roles in biological events such as cell-cell recognition.

A Novel Food Containing LNT II

At present, its organic chemical synthesis method has been developed, including multi-step operations such as protection, deprotection, and glycosylation. In comparison, enzymatic and fermentation techniques to produce LNT II are more attractive options due to their high efficiency and safety. Currently, a novel food containing a part of LNT II is expected to be applied in infant foods, beverages, table sweeteners, whole meal replacements for weight control, food supplements, foods for special medical purposes, and various other foods. This new type of food is also mainly composed of various human milk oligosaccharides, it is obtained through microbial fermentation.

LNT II Production Service at CD BioGlyco

CD BioGlyco mainly provides chemical synthesis, chemical enzymatic, fermentation, and enzymic catalysis to realize the production of LNT II. Our researchers work out some Strategies for LNT II production, including but not limited to:

• Construction of an engineered E. coli cell factory to produce LNT II.
• Introducing heterologous glucanotransferase.
• To increase the supply of the precursor of LNT II, related pathway enzymes such as glucosamine synthase, deacetylase, and pyrophosphorylase, were modulated, optimized, and overexpressed in combination.
• Enzymic catalysis
• Directed evolution and site-specific saturation mutation of glycosidase for further engineering.
• Identification of transporter genes and inhibition or increase of corresponding gene expression.

Advantages of Us

• Optimal enzyme expression levels

Blocking the bypass pathway and modulating the gene expression strength to increase the titer of LNT II significantly.

• Green environmental protection and perspective of sustainable development

The production of LNT II from renewable carbon sources.

• Optimization of strain culture conditions.
• Rich resources of enzyme and bacterial resources.

CD BioGlyco is dedicated to providing an efficient one-stop service to help our clients achieve efficient production of LNT II. If you need any further information, please feel free to contact us.

References

1. Liu, Y.H.; et al. Efficient sequential synthesis of lacto-N-triose II and lacto-N-neotetraose by a novel β-N-acetylhexosaminidase from Tyzzerella nexilis. Food Chemistry. 2020, 332: 127438.
2. EFSA Panel on Nutrition; et al. Safety of lacto-N-neotetraose (LNnT) produced by derivative strains of E. coli BL21 as a novel food pursuant to Regulation (EU) 2015/2283. EFSA Journal. 2020, 18(11): e06305.