With the increasing attention of glycomics, glycans chain enrichment and profiling technologies related to lectin have been widely developed. CD BioGlyco is committed to developing lectin-based technologies for glycans analysis. We look forward to being your partner in the field of glycobiology.

Introduction

Lectins are a diverse group of glycan-binding proteins in organisms. It has the characteristics of reversible binding to glycan chains, no modification of polysaccharides, and no enzyme catalytic activity. They have specific recognition capability for glycans and are often used as a "decoder" to elucidate the detailed structure information of glycan chains on carbohydrate conjugates. Lectins have been extensively used in the field of biochemistry, mainly for the detection and enrichment of glycans and glycoconjugates. The low affinity and broad specificity of lectins are considered to be suitable for "profiling" tasks to cover a wider range of ligands.

Lectin-based glycomics is a new comprehensive technology in post-genomic science. In recent years, CD BioGlyco has developed various lectin-based techniques such as lectin blots, lectin histochemistry/cytochemistry, lectin-antibody sandwich enzyme-linked immunosorbent assay (ELISA), lectin affinity chromatography, lectin microarray, and lectin microfluidics. Combined with the business philosophy of customer first and endeavor to meet customer requirements, we focus on developing fast and sensitive glycan analysis services in combination with international cutting-edge scientific research trends.

Fig.1 Lectin microarray analyses of host cell-specific glycan profiles of the hemagglutinins of influenza A viruses. (Takahiro, 2019)

Services

A variety of lectin-based techniques at CD BioGlyco have been successfully applied to glycoprotein analysis with high sensitivity. Services we provide include but are not limited to:

• Lectin blot is used to detect glycoproteins separated by gel electrophoresis (1D-SDS-PAGE or 2D-SDS-PAGE) and transferred onto membranes for lectin blotting.
• Lectin affinity chromatography was used as a way for targeting particular sub-glycoproteomes from clinical samples for the discovery of cancer-associated biomarkers.
• We use lectin histochemistry/cytochemistry to directly observe the localization and distribution of glycans on glycoproteins in tissues and cells.
• We have developed a new platform represented by a lectin microarray that can perform multiple glycan-lectin interaction analyses. These techniques provide customers with useful clues about the characteristic features of glycoconjugates including differences in terminal modifications (e.g., sialic acid bond, fucosylation type) and differences in higher ordered structures in glycan density, depth, and direction composed for N- and O-glycans.

Applications

• Application in bio-marker investigation
• Research on the involvement of glycans chains in tumorigenesis
• Diagnosis and prognosis evaluation of tumor

Advantages of Us

• Specificity and high sensitivity
• Stability and consistency
• Detailed after-sale service

The development of lectin-based technologies is helpful to analyze the structure of complex polysaccharides rapidly and accurately. With many years of experience, CD BioGlyco provides a complete set of glycoprotein structure analysis services to help customers get landmark development. If you are interested in our services, please contact us for more information.

Reference:

1. Takahiro; et al. Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses. Virology. 2019, 527: 132-140.