Properties
Description
Lichenase from Bacillus subtilis, a specific endo-(1-3),(1-4)-β-D-glucan 4-glucanohydrolase, totally solubilizes β-glucans from cereal grains by attacking β-(1,4) glucosidic linkages to give gluco-oligosaccharides.
Molecular Weight
26.8 kDa
Unit Definition
One unit of lichenase activity is defined as the amount of enzyme required to release one µmole of glucose-reducing-sugar equivalents per minute from barley β-glucan (10 mg/mL) in sodium phosphate buffer (100 mM), pH 6.5 at 40°C.
Applications
Lichenase from Bacillus subtilis can be used in carbohydrate research and biofuel industries.