α-linked Galactose & N-Acetylgalactosamine (GalNAc)-binding Lectin Production Service

Speed Up Project Progress by Optimizing the Service Process

Further research on the structure and functions of Galactose-binding Lectins or based on the development of drugs, a large number of high-purity target recombinant proteins is the top priority. From the selection of the target gene to the characterization of the final purified protein, various factors affect the expression of the target protein.

Our production team provides help and support for every step of your project. We provide several optimized and reliable expression systems to rapidly prepare the α-linked galactose & N-Acetylgalactosamine (GalNAc)-binding lectin. Clients choose the expression system according to their needs with the support of our specialists.

α-linked Galactose & GalNAc-binding Lectin Production Service at CD BioGlyco

• Process services

• Design: Our scientists provide precise primer design services. Meanwhile, we support our clients to provide primers.
• PCR amplification: Our experienced researchers rapidly and specifically amplify the target lectin gene.
• TA clonal lectin production: We use Taq polymerase to utilize the added tail to the 3′ end of the amplified product, enabling efficient ligation with the vector in the TA cloning system.
• To prepare the competent state of E. coli: The TA ligation products are transformed into E. coli for verification to obtain the resistant strain, colony PCR tests the correct clone, and the recombinant plasmid is digested to verify the recombinant plasmid.
• Transformation verification, sequencing, and plasmid preservation: The initially confirmed engineered bacteria will be sequenced. The measured gene sequence is compared with the target fragment sequence for the success of the target gene cloning.
• Initial induced expression of the target protein

Fig.1 Flowchart of induced expression of target proteins. (CD BioGlyco)

• The mass number of purified target proteins

Target proteins are purified using affinity chromatography and salt-ion exchange chromatography. All time, temperature, and buffer concentration purification conditions are optimized to ensure a rapid and efficient purification process.

At CD BioGlyco, we provide two specificity galactose-binding lectin production services. Our scientists enable host-specific expression to complete production through client-specific requirements.

• Specificity: Terminal α-linked Galactose & N-Acetylgalactosamine (GalNAc)
• Specificity: Terminal α-linked Galactose > N-Acetylgalactosamine (GalNAc)

Publication

CD BioGlyco provides our clients with Lectin Production to meet quality and yield needs, accelerate the progress of projects, and improve research results. Please feel free to contact us to inform our staff about your recombinant lectin expression application needs, and to consult the solutions and services we provide.