Mesoporous materials have been widely used for glycopeptide enrichment because of their relatively large specific surface area, strong stability, and abundance of activation sites. CD BioGlyco has developed strategies for glycopeptide isolation and enrichment based on mesoporous materials. We have the confidence to provide customers with scientific assistance in the field of glycoproteomics.

Mesoporous Materials

The isolation and separation of glycopeptides using mesoporous materials have been reported in many glycoproteomics studies. Currently, several types of porous materials exist and are classified into different groups based on size. Macroporous and microporous materials have pore diameters greater than 50 nm and less than 2 nm, respectively, mesoporous materials are in the middle. Mesoporous silica nanomaterials show great promise in selectively capturing small-size targets from complex samples due to their regular pore arrangement, strong stability and easily functionalized inwalls. They are widely used in drug delivery, imaging, biosensors and other fields. Under the impact of strong capillary forces, bio-molecules entering mesoporous pores encounter more binding activation sites, thus enhancing the separation efficiency.

Advantages such as large specific surface area, size exclusion for large molecular weight biomolecules, surface modification potential, and support of strong capillary force at the nanoscale are the reasons for the widespread use of mesoporous materials. With their extensive applicability and adaptability, the use of mesoporous materials will have a sustained impact on the development of glycoproteomics.

Fig.1 The flow chart of the enrichment process for glycopeptides by using Fe₃O₄@mSiO₂-IDA nanomaterials. (Sun, 2017)

Our Strategies

CD BioGlyco has established several effective solutions for the enrichment process of glycopeptides to increase the concentration of glycopeptides while removing other constituents. The strategies we offer include, but are not limited to:

• Mesoporous silica materials functionalized with boronic acid for the detection of glycopeptides. In this method, glycopeptides in solution bind with high affinity to the groups attached in the pores, significantly improving the solute detection limit.
• Enriched N-linked glycans by oxidized and ordered mesoporous carbon materials of CMK-3 followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS, which allows the profiling of N-linked glycans in complex samples.
• Iminodiacetic acid (IDA) groups are grafted onto the inwalls of magnetic mesoporous silica (mSiO₂) nanomaterials for highly specific enrichment of N-linked glycopeptides.

Applications

• Research on evolution and progression in tumor
• Study on the relevance between glycosylation and disease
• Diagnosis and monitoring of diseases
• Biomarker discovery

Advantages of Us

• Simple preparatory methods and low cost
• Strict quality control
• Experienced and highly qualified talents
• Global leading instrumentation and software system

CD BioGlyco has established friendly partnerships with many world-renowned enterprises, universities, and scientific research institutions, and provided them with high-quality services in the field of glycomics. Our services have been unanimously recognized by our customers. If you need scientific assistance, please contact us for further consultation.

Reference

1. Sun, N.; et al. Hydrophilic mesoporous silica materials for highly specific enrichment of N-linked glycopeptide. Analytical Chemistry. 2017, 89(3):1764-1771.