Glycosphingolipids (GSLs) play a key role in the manifestation of some diseases because many pathogens target GSLs as membrane receptors for colonization. CD BioGlyco has a first-class experimental platform and experienced researchers to provide customers with optimized microorganism GSL extraction service.

Biological Significance of GSL

GSL is formed by a ceramide backbone linked to one or more glycosyl groups. It contains two main components, namely the hydrophobic lipid chain as well as the hydrophilic glycoconjugate chain. Such a structure is required to extend the polar end of the glycoconjugate component outside the cytoplasmic membrane after embedding it in the lipid bilayer of the cell membrane. The presence of more than 400 GSLs has been identified based on the structural identification of glycan chains, and the structure of its ceramide is variable. The characteristics of GSL metabolism and composition change during the proliferation and differentiation of various cell types, suggesting a correlation between the GSL expression pattern and the function of these cells.

Fig.1 Structure of ceramide. (Wikipedia)

Microorganism GSL Extraction Services

The laboratory at CD BioGlyco uses the most advanced extraction technology to provide customers with the customized extraction of microorganism GSL. The services we provide include but are not limited to:

• Microorganism GSL extraction services based on acid hydrolysis extraction

Our services enable efficient extraction of target compounds from complex mixtures and are suitable for a wide range of microorganisms such as bacteria, fungi, algae, etc.

• Microorganism GSL extraction services based on ultrasonic-assisted extraction

High extraction rates can be obtained by using the extreme pressure generated by ultrasonic cavitation in this service. Compared to traditional solvent extraction methods, our service uses water or other non-organic solvents that do not cause irreversible damage to microbial cell walls and membranes.

• Microorganism GSL extraction services based on enzymatic extraction

This service decomposes raw materials through enzyme reaction, thus reducing the difficulty of extraction, which is conducive to the dissolution of polysaccharides in the cell wall, and improves the extraction rate of products.

Fig.2 The extraction technologies at CD BioGlyco. (CD BioGlyco,2023)

Applications

• Emulsifiers and stabilizers in foods
• Emulsifiers and surfactants for biodiesel and bio-kerosene

Advantages of Us

• High extraction rates
• Do not cause irreversible damage to microbial cell walls and membranes
• High purity and very low contaminating nucleic acids and proteins
• The activity and structure of the product are more stable

CD BioGlyco has advanced extraction technology and powerful tools for data processing and analysis to provide high-quality, reliable, and courteous microbial GSL extraction services to our clients. If you are interested in our services, please feel free to contact us for more details.

Reference

1. Wang, W.; et al. Polysaccharides from fungi: A review on their extraction, purification, structural features, and biological activities. Food Chemistry: X. 2022: 100414.