Modular Synthesis-based Oligosaccharide Library Construction Service

Unraveling the Mystery of Oligosaccharide Library Construction with Precision and Ease

One of the most important functions of an oligosaccharide library is to develop oligosaccharide microarrays, so its construction is very important. At CD BioGlyco, we provide Oligosaccharide Library Construction Services on top of the development of glycan microarrays. Among them, the assembly of oligosaccharide library using modularization is as follows.

Firstly, we use monosaccharides or disaccharides as glycosylated donors and prepare tri-, tetra-, and other oligosaccharides by glycosylation. In this process, we provide chemical modifications such as selective sulfation, sulfonation, acetylation, or hydrogenation to extend the backbone structure of the oligosaccharides.
Then, we utilize glycosyltransferases to modify the oligosaccharides prepared in the previous step to further extend the structure and type of oligosaccharides and synthesize oligosaccharide fragments efficiently.
Next, oligosaccharide units with different main chain structures or different branched structures are assembled efficiently, respectively. Among them, we provide chemical reagents to regulate and realize the construction of glycosidic bonds with high stereoselectivity.
Finally, we utilize an orthogonal one-pot glycosidation reaction to complete the assembly of the target oligosaccharide library.

Throughout the process, we offer nuclear magnetic resonance (NMR), high-performance liquid chromatography (HPLC), and electrophoresis to analyze the structure of the oligosaccharides. We also offer weak anion exchange column chromatography to remove impurities and unreacted substances.

Fig.1 Processes of modular synthesis to construct oligosaccharide libraries. (CD BioGlyco)

Publication

Technology: Modular disaccharide assembly of tetrasaccharides with selective protection

Journal: Journal of the American Chemical Society

IF: 15.0

Published: 2017

Results: The authors describe a synthetic method for the construction of heparan sulfates (HS) oligosaccharide libraries. The structure is based on the use of modular disaccharides to form selectively protected tetrasaccharides. These tetrasaccharides have been subjected to selective chemical modification such as regioselective O- and N-sulfation and selective desulfation and enzymatic modifications. A total of 12 different HS derivatives were obtained. A library of 47 HS oligosaccharides has been developed and the resulting compounds have been synthesized to create HS oligosaccharide microarray, using tetrasaccharide backbone compositions with various compositions. The ligand requirements of many HS-binding proteins, such as fibroblast growth factor and chemokines, were examined using this array.

Fig.2 Modular synthesis of HS oligosaccharides. (Zong, et al., 2017)

Applications

Oligosaccharide libraries based on modular synthesis can be used to develop methods for the rapid preparation of oligosaccharides with different biological activities.
Oligosaccharide libraries based on modular synthesis can be used to prepare oligosaccharide microarrays.
Oligosaccharide libraries based on modular synthesis facilitate the rapid preparation of diverse oligosaccharides.

Advantages

We have first-class oligosaccharide biosynthesis technology and provide clients with a variety of oligosaccharide library construction solutions.
We have rich experience and knowledge base in the construction of oligosaccharide libraries to ensure the accuracy of oligosaccharide libraries.
We not only provide the construction of oligosaccharide libraries but also provide high-quality glycan microarray construction and Assay services.

CD BioGlyco has a powerful Glycan Display Platform, providing clients with oligosaccharide library construction as well as glycan microarray detection in a one-stop service. Each of our protocols is developed by experts in the field of glycobiology and has passed quality tests. At the same time, our oligosaccharide libraries or glycan microarrays are highly stable and flexible. If you are interested in our oligosaccharide library-related services, please feel free to contact us.

Reference

Zong, C.L.; et al. A heparan sulfate microarray reveals that heparan sulfate-protein binding exhibits different ligand requirements. J. Am. Chem. Soc. 2017, 139(28): 9534-9543.

This service is for Research Use Only, not intended for any clinical use.