At CD BioGlyco, our professional research teams allow us to provide clients with high-quality Lipidomics Analysis Services and integrated Sphingolipid Analysis Services. Our advanced technology platforms and high-quality services will fully meet your needs.
GM2 represents a heterogeneous autosomal recessive group of disorders caused by deficiency of the lysosomal enzyme β hexosaminidase. The resulting accumulation of ganglioside GM2 occurs primarily in neuronal cells and coincides with a progressive broad spectrum of neurologic deterioration. The classic infantile form is known to lead to the death between 3 and 5 years of life, but the rate of functional decline remains poorly defined. The juvenile- and adult-onset variants of the hexosaminidase deficiencies have a later onset, slower progression, and more variable neurologic findings. Therefore, it is crucial to use accurate and simple quantitative methods for GM2 analysis.
Fig.1 Structure of GM2. (Wikipedia)
CD BioGlyco provides comprehensive Monosialoganglioside (GM) Analysis Services utilizing a variety of technologies. We offer a tandem mass spectrometry (MS/MS) method for measuring GM2 in samples. The method employs a tandem mass spectrometer equipped with an HPLC system. The analysis is performed using the isotope dilution method with a deuterium-labeled internal standard. After the sample is deproteinized by mixing with an internal standard solution and centrifuge, the supernatant is injected into a C-18 column. After a few minutes of purging, the switching valve is activated and the analytes are eluted from the column through a water/methanol gradient into the MS/MS system. Quantification by multiple reaction monitoring (MRM) analysis is performed in negative mode. This method is also used to simultaneously quantify GM1 and GM2 in samples. Taking human cerebrospinal fluid patient samples as an example, our analysis process is as follows.
Stock solutions of GM2 and internal standards are prepared separately in methanol. In-house quality control solutions at three concentrations are prepared in the same way to evaluate within-day and between-day precision as well as the accuracy of the method.
Standards, quality control samples, or samples are mixed with an internal standard solution for deproteinization. After vigorous vortexing followed by centrifugation, the resulting supernatant is injected into the LC-MS/MS system. For samples with concentrations higher than the standard calibration range, the sample volume should be appropriately reduced.
The injected sample is extracted/cleaned online and then introduce into the mass spectrometer by activating a built-in switching valve.
The accuracy of this method is evaluated by performing recovery studies. The between-day and within-day precision is assessed by analyzing in-house quality control samples at three different concentrations in replicates for GM2.
CD BioGlyco provides systematic overall solutions for Ganglioside Analysis. Our broad vision provides customers with professional and high-quality help for GM2 analysis. If you are interested in our services, please contact us for more details without any hesitation.
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