CD BioGlyco has top talents who provide comprehensive Lipidomics Analysis Services. We have rich experience to analyze Sphingolipids according to our clients' detailed requirements by various methods. Our professional research teams provide customers with high-quality GM4 analysis services according to your needs.

Overview of Monosialoganglioside 4 (GM4)

GM4 is a monosialoganglioside found mainly in the central nervous system and was found to be a major component of myelin gangliosides. It has also been identified as a specific marker for human myelin and oligodendrocyte nuclei. In the chicken cerebellum, GM4 is associated with astrocytes but not myelin. The appearance of GM4 in extraneural tissues was first reported in egg yolk and chicken thymus. This ganglioside has also been detected in mouse erythrocytes, rat kidneys, chicken embryo liver, chicken liver, and frog liver. Among these tissues, GM4 was found to be the major ganglioside in egg yolk, chicken embryo liver, and frog liver.

Fig.1 Structure of GM4. (Pubchem)

GM4 Analysis Service at CD BioGlyco

CD BioGlyco provides client-tailored Monosialoganglioside (GM) Analysis Services with rapid analysis procedures and easy to read report, to speed up your scientific research. The structures of sugar chains and ceramide moieties of GM4 are analyzed by chemical analysis combined with chromatographic analysis, mass spectrometry (MS), and nuclear magnetic resonance spectroscopy (NMR).

• The chemical analysis combined with chromatographic analysis

Lipid-bound sialic acid is analyzed by the resorcinol-HCI method using NeuAc as a standard. The extracted sialic acid is analyzed by GC and GC/MS.

• Secondary ion mass spectrometry and collision-induced dissociation mass spectrometry

Secondary ion mass spectrometry (SIMS) spectra of gangliosides are recorded on a quadrupole mass spectrometer in negative and positive ion modes. The sample dissolved in chloroform-methanol is loaded onto the SIMS target tip, analyze by the program and a scan is acquired to obtain a spectrum.

• NMR

For NMR analysis, ganglioside is exchanged with D₂O, lyophilize, exchange again with CD₃OD, and evaporate to dryness. The ¹H-¹³C correlation of the heteronuclear single bond is detected, and the ¹H heteronuclear multiple bond linkage is detected.

Advantages of Us

• GM4 analysis utilizes a variety of efficient methods, including chemical analysis combined with chromatographic analysis, mass spectrometry, and nuclear magnetic resonance spectroscopy.
• A comprehensive and advanced platform.
• Experienced and well-trained technicians.
• Comprehensive and reliable after-sales service.

CD BioGlyco devotes to high-quality Ganglioside Analysis Services and provides many solutions. We have provided various high-quality and efficient services for many clients from different countries. If you are interested in our services, please contact us for more details without any hesitation.

Reference

1. Li, Y.T.; et al. Association of GM4 ganglioside with the membrane surrounding lipid droplets in shark liver. Journal of lipid research. 2002, 43(7): 1019-1025.