Properties
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25 °C.
Form
ammonium sulfate suspension
Purity
ammonium sulfate suspension, ≥10 units/mg protein
Applications
β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides, and also can be used for chemical synthesis of oligosaccharides.It may be a useful tool for studying Alzheimer's disease. Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms .
