N-linked Glycan Pathway-based Glyco-engineered Cell Construction Service

Enhancing Cells, Elevating Potential: Navigating N-Linked Glycan Pathways

CD BioGlyco has a systematic platform for Glycan Display and provides various services related to cell construction as well as the construction of glycan arrays. Based on the glycosylation pathway of N-linked glycans, CD BioGlyco provides corresponding Glycan Engineering Cell Construction Services.

To expand the application of the constructed cell lines, we utilize the HEK293 cell line for the construction of glycoengineered cells. At the same time, we analyze the glycosylation pathway and the expression of related genes in the cell lines using glycol/genetic engineering.
To target the glycosyltransferase gene dedicated to the N-linked glycan pathway, we add it to the HEK293 cell line using RNA sequencing genomics and gene "knock-in (KI)" and "knock-out (KO)" means. Then, we use targeted KI integration of the glycosyltransferase gene to introduce an N-glycan signature that is not endogenously expressed in HEK293 cells and flow cytometry to test the expression of the glycosyltransferase gene.

In addition, we provide combinatorial approaches to eliminate and introduce de novo glycosylation capabilities to develop a sub-library of stably engineered HEK293 homozygous cells that individually show loss or gain of different features of the N-glycosome.

Fig.1 N-linked glycan pathway-based glyco-engineered cell construction service. (CD BioGlyco)

Publication Data

Technology: Precision genetic engineering, mapping of glycosylation pathways

Journal: Molecular Cell

IF: 16.0

Published: 2019

Results: Using glycosylation pathway mapping, the authors generated a library of homozygous HEK293 cells with combinatorially engineered glycosylation capacity, displaying and dissecting the genetic, biosynthetic, and structural basis of glycan binding in the natural environment. Mapping of glycosylation pathways organizes glycosyltransferase genes into pathway-specific and non-pathway-specific steps in the biosynthesis of different glycoconjugates and illustrates the potential redundancy of individual biosynthetic steps provided by isozymes and other competitive biosynthetic steps. The maps provide predictions of structural glycan features influenced by the presence or absence of individual genes, and whether global or differential nuances are expected.

Fig.2 Glycosylation pathway of N-linked glycans. (Narimatsu, et al., 2019)

Applications

Glyco-engineered cell constructs can be used to characterize glycan groups by genetic methods.
Glyco-engineered cell constructs can be used to analyze the genetic, biosynthetic, and structural basis of glycan binding.
Glycoengineered cell constructs can be used to probe the glycan binding specificity of lectins and microbial adhesins, and can also be used to profile the glycan binding specificity of different influenza hemagglutinins.

Advantages

We have high-quality products and perfect services, which make our clients save their efforts.
We have an expert R&D team to provide first-class technology.
With the comprehensive technical advantages of multi-platform and upstream/downstream integration, we provide custom cell construction services to clients.

CD BioGlyco is a company dedicated to research in the field of glycobiology. With our world-leading biology technology, we have constructed a powerful glycan display platform to provide custom glycan display services to clients all over the world. Our excellent service and high-quality products have won the praise of our clients. If you are interested in our services, please feel free to contact us.

Reference

Narimatsu, Y.; et al. An atlas of human glycosylation pathways enables display of the human glycome by gene engineered cells. Molecular Cell. 2019, 75(2): 394-407.

This service is for Research Use Only, not intended for any clinical use.