Nanoparticles are widely used for the isolation and separation of glycopeptides due to their nano dimensionality, tightly controlled size, and distinctive optical and electrochemical properties. CD BioGlyco has developed a variety of particle-based materials based on their properties to provide scientific assistance to customers in the field of glycoproteomics.

Nanoparticles

Nanoparticles that are widely used in assembling chips for biomolecule enrichment have the following characteristics: 1) nanoparticles with various size distributions and morphologies can be synthesized; 2) nanoparticles can be modified and diversified by a variety of reagents for specific applications; 3) they display unique optical and electrochemical properties.

In general, nanomaterials use various interactions between different functional groups bonded on the surface of various carriers and glycopeptides to directly enrich glycopeptides in the sample solution, which shortens the analysis time and reduces sample loss. This strategy not only simplifies the experimental procedure but also improves the sensitivity of the material for the detection of target glycopeptide chains, which is superior for the analysis of trace glycoproteins/glycopeptides. Moreover, the large surface area and easy surface modification of the nanomaterials facilitate the capture of the target glycopeptide chains.

Fig.1 Schematic illustration of glycopeptide capturing using Au25 gold nanoparticles. (Tran, 2012)

Our Strategies

CD BioGlyco has established various glycoprotein/glycopeptide separation and enrichment approaches based on detonation nanodiamond (DND), magnetic nanoparticles (MNPs), polymer nanoparticles, gold nanoparticles(GNPs), SiO nanoparticles as the carriers. We provide our clients with several efficient strategies for glycopeptide enrichment, including but not limited to:

• We typically employ core-shell structured nanoparticles to enrich glycopeptides in complex samples, modifying the outer layers with various organic or inorganic reagents to identify biomarkers of interest.
• We fabricate aminophenyl boronic acid-functionalized magnetic nanoparticles for selective capture of glycopeptides from mixtures containing non-glycomolecules.
• We use a layer-by-layer method to synthesize MNPs, which are coated by multilayered polysaccharide shells and we employed these hydrophilic materials to selectively enrich glycopeptides from biological samples.
• We have developed a novel gold nanoparticle functionalized with ultra-small hydrazide groups capable of isolating 90% of the glycopeptides from complex biological samples.

Applications

• Application in the identification of N-glycosylation sites
• Application for characterizing protein glycosylation
• Applications in glycoproteomics for biomarker detection
• Applications in early diagnosis of diseases

Advantages of Us

• High specificity and high separation
• Optimize strategies according to customers’ requirements
• Customer-centered service
• Detailed after-sales service

CD BioGlyco continues to work on the development and synthesis of new affinity materials to solve the problem of isolation and enrichment of low abundance glycoproteins/glycopeptides from complex biological samples. If you are interested in our services, please feel free to contact us for further inquiries.

Reference

1. Tran, T.H.; et al. Ultrasmall gold nanoparticles for highly specific isolation/enrichment of N-linked glycosylated peptides. Analyst. 2012, 137 (4): 991-998.