CD BioGlyco is experienced in Cell Surface Glycoengineering Based on Salivary Acids. Neu4Ac is a positional isomer of Neu5Ac. Sialic acid plays an important role in the biological activity of glycoconjugates, such as cellular recognition and communication. Some glycoconjugates are harmful to organisms. It is important to prepare salivary acid analogs as potential inhibitors. In order to meet the different research needs of our clients based on salivary acids, we provide targeted introduction and analytical services for Neu4Ac and its derivatives.
Fig.1 Types of Neu4Ac-based cell surface glycoengineering service. (CD BioGlyco)
We use chemical and enzymatic modifications to target Neu4Ac. We have a complete Glycoengineering Platform. With rich experience in chemosynthesis, we perform O-acetylation at the C-4, C-7, C-8, and C-9 positions of the hydroxyl group of sialic acid. In addition to this, we employ Gene Editing to target the introduction of Neu4Ac, including gene overexpression or knockout. By regulating the expression of Neu4Ac synthesis-related genes, we can affect the structure of Neu4Ac and ultimately the corresponding physiological processes.
Sialic acid is important for the conformation and stability of glycoproteins and is involved in a variety of interactions. structural analysis of Neu4Ac plays a crucial role in understanding its function. We provide Neu4Ac quantitative analysis service. We release and isolate salivary acids from salivary acid glycoconjugates and characterize them. The cleavage of salivary acid consists of two main methods: enzymatic hydrolysis and acid hydrolysis. We mainly use acid hydrolysis to release sialic acid. The released salivary acids can be detected by high-performance liquid chromatography or ultra-high-performance liquid chromatography after labeling.
Technology: Organic Synthesis
Journal: Organic & Biomolecular Chemistry
IF: 3.412
Published: 2010
Results: In this study, two synthetic routes to iso-Neu4Ac are presented. The first method involves the reaction of benzylamine (BnNH2) as a nitrogen nucleophile with α,β-enone. The second method involves the reaction of trimethylsilyl azide (Me3SiN3) as a nitrogen nucleophile. 1,4-Conjugate addition of both BnNH2 and Me3SiN3 to α,β-enone was achieved with moderate synthetic selectivity. The results of the synthesis showed that the product obtained by the first method was impure. The second method gives purified iso-Neu4Ac.
Fig.2 Synthesis of iso-neuraminic acid iso-Neu4Ac by means of thiazolyl phosphorane. (Dondoni, 2010)
CD BioGlyco is experienced in salivary acid glycoengineering. Our goal is to provide accurate and efficient Neu4Ac-based cell surface glycan engineering services to our clients. Please feel free to contact us for more information about Neu4Ac synthesis, specific steps for quantitative analysis, prices, etc. We are ready to provide you with Neu4Ac-based glycoengineering services at any time.
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