Nuclear magnetic resonance (NMR) spectroscopy, as the most effective method of resolving the structure of substances, has promoted the structural and conformational analysis of polysaccharides. CD BioGlyco is dedicated to developing a high-sensitivity and high-resolution NMR solution to provide customized NMR analysis services of polysaccharide-based biodegradable materials for researchers in the scientific and pharmaceutical industries, ranging from sample processing to NMR data acquisition and analysis.

NMR Analysis

The large molecular weight of polysaccharides, the large variety of monosaccharide residues and substituent groups, and the diversity of ways in which monosaccharide residues are linked lead to many difficulties in the structural study of polysaccharides. NMR spectroscopy is one of the most important and useful techniques for the structural identification of polysaccharides and has been widely used for the characterization of polysaccharides, including quantitative analysis, degradation analysis of polysaccharides, the interaction between polysaccharides and other substances, and carbohydrate impurities profiling. This helps to provide valuable information for Carbohydrate-based Biodegradable Material Development.

The principle of NMR is that the absorption of electromagnetic waves from one spin energy level to another by the atomic nucleus in an external magnetic field creates an absorption spectrum that represents structural information about the individual nucleus and its surroundings, allowing the detection and analysis of information such as the heterotopic conformation, glycosidic bonding pattern and linkage order of polysaccharides. The molecular structure of polysaccharides needs to be analyzed with the aid of 2D NMR techniques because of the significant signal overlap of identical atoms in 1D NMR.

Fig.1 The chemical shifts of polysaccharides in 1D NMR spectra: a) ¹H NMR spectrum, b) ¹³C NMR spectrum. (Wang, et al., 2021)

NMR Analysis of Polysaccharide-based Biodegradable Materials at CD BioGlyco

We offer 1D NMR (¹H and ¹³C NMR) and 2D NMR (correlation spectroscopy(COSY), total correlated spectroscopy (TOCSY), nuclear overhauser effect spectroscopy (NOESY), heteronuclear single-quantum correlated spectroscopy (HMQC), heteronuclear single-quantum correlated spectroscopy (HSQC), and heteronuclear multiple-quantum correlated spectroscopy (HMBC)) to help our clients analyze polysaccharide-based materials. Our specialized Chemical Characterization of Polysaccharide-based Biodegradable Materials allows us to provide our clients with information such as the monosaccharide composition of the polysaccharide, the order of monosaccharide residues, and the configuration of the glycosidic bond. Our services include but are not limited to:

• We provide structural NMR analysis of polysaccharide-based materials using polysaccharide 1D and 2D NMR techniques, combined with data on monosaccharide composition and polysaccharide methylation analysis.
  • Quantitative analysis of the relative content of sugar residues
  • Analysis of the configuration of sugar rings
  • Analysis of the glycosidic bonds and their conformation
  • Analysis of the connection mode and order of glycosyl
  • Analysis of the substitution functional groups and substituent positions
• Determination of the absolute purity of polysaccharide-based materials.
• Chemical exchange analysis and molecular mobility analysis.
• A complete NMR analysis report, including the process of NMR resolution of polysaccharides, attribution, and labeling of chemical shifts of sugar residues, high-resolution NMR processing of spectra (e.g. spectral peak processing), and inferred structural models.

Fig.2 Workflow of NMR spectroscopy services of polysaccharide-based biodegradable materials. (CD BioGlyco)

CD BioGlyco has many years of experience in the Characterization of Polysaccharide-based Biodegradable Materials. Our high-resolution NMR spectrometers and excellent data processing speed and storage capacity for 1D, 2D, and multidimensional spectra can support our clients in deducing the structure of polysaccharide-based materials.

If you are interested in our NMR analysis, please feel free to contact us for more details.