Non-natural GalNAc Analog-based Cell Surface Metabolic Oligosaccharide Engineering Service

Non-natural GalNAc Analog-based Cell Surface Metabolic Oligosaccharide Engineering Service at CD BioGlyco

CD BioGlyco has been working on the research of Cell Surface metabolic oligosaccharide engineering (MOE) for many years. Structural modification of proteins is essential for normal physiological activities of cells. Their structural changes often cause diseases. Glycans are one of the main causes of structural variation. The study of glycans and their interactions with carbohydrates, proteins, etc. MOE is expected to drive breakthroughs in medical treatment, etc., and is a proven technology that can used in this research. We offer cell surface MOE services based on non-natural GalNAc analogs.

Non-natural sugar analogs can be used as chemical probes for MOE. First, we design and synthesize non-natural GalNAc analogs containing azide groups, etc. After that, we treat cells with them. Through the endogenous glycan biosynthesis mechanism of the cells, the labeled monosaccharides are then introduced into the desired glycan. Through MOE, moieties including ketones, azides, etc. are added to the glycoconjugates, thus providing chemical labeling. The introduced label can be further labeled by bioorthogonal click reactions, ultimately enabling visualization, enrichment, and/or modulation of the glycan structure.

Fig.1 Process for MOE services based on non-natural GalNAc analogs. (CD BioGlyco)

Publication

Paper Title: 4-Deoxy-4-fluoro-GalNAz (4FGalNAz) is a metabolic chemical reporter of O-GlcNAc modifications, highlighting the notable substrate flexibility of O-GlcNAc transferase

Technology: Stereochemistry

Journal: ACS Chemical Biology

IF: 4

Published: 2022

Results: Metabolic chemical reporters (MCRs) are used to identify and characterize different glycans. In order to make GalNAc-based MCRs more selective, this study attempted to modify GalNAc residues. A new MCR, Ac₃4FGalNAz, was synthesized in nine steps, and cells were treated with Ac₃4FGalNAz. It was found to be able to label a variety of proteins. It modifies proteins mainly by O-linkage and does not inhibit O-GlcNAc or glycosaminoglycans.

Fig.2 Ac₃4FGalNAz treatment results in protein labeling in live cells. (Jackson, et al., 2022)

Applications

• Cell surface MOE based on non-natural GalNAc analogs can be used to characterize glycan structures and monitor dynamic changes in glycan levels.
• Glycans are recognized as key players in a range of pathological processes. Cell surface MOE based on non-natural GalNAc analogs can be used to study surface markers associated with diseases.
• Cell surface MOE based on non-natural GalNAc analogs can be used for drug delivery studies.

Advantages

• GalNAc-based MCRs may not be selective for different classes of glycans. We make various modifications to GalNAc. We aim to synthesize non-natural GalNAc analogs that are more selective, thereby expanding the range of applications for MOE.
• Our non-natural GalNAc analogs are diverse. We offer MOE services for a wide range of studies.
• We provide high-quality cell surface MOE services based on non-natural GalNAc analogs, which can help our clients characterize glycans and regulate their functions in the natural cellular environment.

CD BioGlyco has a complete Glycoengineering Platform, which provides Cell Surface Metabolic Glycoengineering Service, MOE service, etc. We customize the glycoengineering program according to your requirements. Please feel free to contact us for more information about non-natural GalNAc analogs, MOE experimental procedures, etc.