Overview of Deoxyuridine

Deoxyuridine is a nucleoside, which is also called pyrimidine 2'-deoxyribonucleosides. Deoxyuridine exists in all living organisms, ranging from bacteria to humans. Biosynthesis can be used to produce deoxyuridine by its interaction with the enzyme cytidine deaminase.

Fig.1 Synthetic route to deoxyuridine. (Romero-Perez, et al., 2019)

Nucleoside-based Deoxyuridine Production Service at CD BioGlyco

CD BioGlyco is a professional Nucleoside Production servicer, with proficient problem-solving skills and a high success rate, we provide clients with a high-quality nucleoside-based deoxyuridine production service.

• Production methods
  
  • Chemical synthesis
    
    • The solid-supported phosphoramidite method.
      
      • The dehydration reaction of ribose together with the corresponding purine base is acted in the presence of inorganic polyphosphate salts.
  • Biosynthetic methods
    
    • We demonstrate that deoxycytidine could be readily achieved by recombineering with biosynthetic genes and regulatory genes. In addition, we show that the methods efficiently improved deoxyuridine yield and decreased byproduct content.
• Production capacity
  
  • We achieve large-scale production and provide bulk-purified deoxyuridine.
• Quality control analysis
  
  • The incorporation of ultra-violet (UV) spectroscopy with high-performance liquid chromatography (HPLC) assay (HPLC-UV)
    
    • HPLC-UV has been validated for the determination of deoxyuridine content and purity.
  • Liquid chromatography-mass spectrometry (LC-MS)
    
    • LC-MS allows the separation of nucleosides based on their physicochemical properties, such as polarity, hydrophobicity, and size.
  • Gas chromatography-mass spectrometry (GC-MS)
    
    • GC-MS involves the separation of volatile or derivatized nucleosides by gas chromatography followed by their detection and identification using mass spectrometry.
  • High-resolution mass spectrometry (HRMS)
    
    • HRMS provides enhanced mass accuracy and resolution, enabling the precise determination of nucleosides and their isotopologues.
  • Tandem mass spectrometry (MS/MS)
    
    • MS/MS offers structural information about nucleosides and their modified forms.
• Structure modification
  
  • Modification of deoxyuridine structure according to the specific needs of clients' research and project development to obtain products with different uses and functions.

Fig.2 Nucleoside-based deoxyuridine production. (CD BioGlyco)

Applications

• Deoxyuridine is widely used in molecular biology, pharmacology, and other scientific research.
• Deoxyuridine serves as a precursor for nucleotide synthesis.
• Deoxyuridine is used to develop a short DNA detection method using a deoxyuridine probe.

Advantages

• Mass spectrometry-based analytical techniques enable sensitive and comprehensive detection, identification, and quantification of nucleosides and their modified forms in complex biological samples.
• Our deoxyuridine production service is more time-saving, the production cycle is shortened, and the use of toxic reagents is reduced.
• We process detailed after-sales service.
• The product is high purity and environmentally friendly.

CD BioGlyco has a professional nucleoside production team to provide efficient and fast production services. For additional information, please feel free to contact us.