Structure and Properties of Thymidine

Thymidine is classified as a pyrimidine nucleoside, which is linked by the pyrimidine base thymine, also called deoxythymidine, deoxyribosylthymine, or thymine deoxyriboside, which belongs to a pyrimidine deoxynucleoside. Thymidine serves as a substrate for thymidine kinase EC2.7.1.21, which phosphorylates nucleosides with phosphate from ATP. Thymidine pairs with deoxyadenosine (A) in double-stranded DNA at the DNA nucleoside T. RNA synthesis involved no precursors of thymine nucleotides. Thymidine exists exclusively in DNA and the T-loop of tRNA. dTMP, dTDP, or dTTP can be created by being phosphorylated with one, two, or three phosphoric acid groups of deoxythymidine. It exists as small white crystals or white crystalline powder in solid form.

Fig.1 Thymidine and five approved thymidine nucleos(t)ide analogues. (Li, et al., 2021)

Nucleoside-based Thymidine Production Service at CD BioGlyco

CD BioGlyco currently has experience with many projects of thymidine production, and help you develop your project for better application in pharmaceutical, chemical, and other fields. We provide a large variety of synthesis scales, modifications, and purifications to satisfy your various needs.

• Production methods
  
  • Chemical synthesis
    
    • The solid-supported phosphoramidite method is the gold standard method for thymidine synthesis.
  • Biosynthetic methods
    
    • A metabolic engineering approach is used to develop a thymidine-producing strain with a relatively high yield.
• Quality control analysis
  
  • The incorporation of ultra-violet (UV) spectroscopy with high-performance liquid chromatography (HPLC) assay has been validated for the determination of thymine content and purity.
• Production capacity
  
  • We achieve large-scale production and provide bulk-purified deoxyuridine.
• A variety of modified structures
  
  • Modification of thymidine structure according to the specific need of clients' research and project development to develop to enhance or alter the natural properties of nucleic acids, such as iododeoxyuridine, azidothymidine (AZT), thymidine (3H-TdR), bromodeoxyuridine (BrdU), 5-ethynyl-2´-deoxyuridine (EdU) and so on.

Fig.2 Nucleoside-based thymidine production. (CD BioGlyco)

Applications

• Use in scientific research
  
  • Thymidine hypermodification is used to study chemical diversity.
  • Thymidine is used to study the distribution, specificity, and kinetics of the thymidine kinase family.
• Analysis of DNA
  
  • Thymidine is widely used in the analysis of DNA replication, DNA repair, and cell proliferation.
• Thymidine incorporation assay
  
  • Thymidine Incorporation assay is the most reliable assay to measure cell proliferation.

Advantages

• High quality and stability of products.
• Short production cycle.
• Professional after-sales service.
• Reducing the use of toxic reagents and less pollution.

CD BioGlyco has been deeply involved in Nucleoside Production for many years. Our goal is to help you speed up the production process and provide custom nucleosides. If you are interested in our services, please feel free to contact us.