Safeguarding the Foundations of Your Research with Oligosaccharide Library Immobilization

Oligosaccharide libraries or related other molecules can be immobilized on different types of solid phase supports for use in affinity purification of oligosaccharides libraries, analysis of samples, and subsequent processing and applications. CD BioGlyco provides different oligosaccharide library immobilization services according to the client's needs.

• We select slides as the immobilization phase and print different oligosaccharide libraries using contact printing or piezoelectric (non-contact) printing techniques.
  • We print a small amount of the oligosaccharide solution as a spot on the surface of the slide.
  • The slides are incubated, causing the oligosaccharide samples to become immobilized on the slide surface.
  • We enclose the slides to prevent non-specific binding of chemicals to the oligosaccharides. At the same time, slides containing oligosaccharide samples are covered with a specific protein buffer and incubated to cause equilibrium binding.
  • We wash the slides to remove unbound proteins and analyze them. In this process, we fluorescently label the bound proteins in advance to facilitate subsequent fluorescence detection.

Fig.1 Process of oligosaccharide library immobilization. (CD BioGlyco)

• In addition, we provide specific oligosaccharide library immobilization services that include, but are not limited to, the following:
  • Glycan coupling to N-hydroxysuccinimide (NHS)-activated glass slide
  • Glycan coupling to epoxide-activated glass slide
  • Glycolipid and lipid-derivatized glycan deposited onto nitrocellulose-coated slide
  For the former two, we utilize chemical methods to couple the free amine at the reduced end of the oligosaccharide to the slide so that it is immobilized on the slide. At the same time, we examine the slides using oligosaccharide-binding proteins after cleaning the unattached material. The assay consists of a direct fluorescence assay and a fluorescence-labeled auxiliary reagent assay.
  For the third, we use nitrocellulose microarrays to deposit oligosaccharide libraries onto nitrocellulose-coated slides in a noncovalent manner and block them appropriately to reduce nonspecific binding.

Publication

Applications

• Oligosaccharide library immobilization can be used to detect biomolecules such as proteins, lipids, and other biomolecules that bind specifically or non-specifically to specific oligosaccharides.
• Oligosaccharide library immobilization can be used to develop microarrays for evaluating specific glycans.

Advantages

• CD BioGlyco has a specialized and comprehensive Glycan Display Platform that provides different glycan display array development services to meet our clients' scientific needs.
• With advanced biological and chemical technologies, we provide accurate and high-quality glycan display array development programs. We also ensure the reliability of each program.

With world-leading glycobiology technology, CD BioGlyco has constructed a comprehensive glycan display platform to provide high-quality glycan display research and development services to every client. All of our programs are developed by experts in biology and chemistry and have passed strict quality tests. If you would like to know more about our services, please feel free to contact us.