Glycosphingolipid (GSL) is a complex substance composed of sphingosine and lipid moieties linked by ester or glycosidic bonds. It is widely found in animal and plant cell membranes and has important roles in the nervous system and immune systems. CD BioGlyco provides our customers with high-quality and hassle-free organic solvent-based purification service.
GSLs are a complicated and diverse class of molecules, and different types of GSLs differ in their compositional structures and biological roles, but in general, they are all important for life activities. In the purification of products using organic solvents, they can be used as separation media to separate the target compounds from the mixture by differences in solubility and volatility of the compounds. This method is a popular approach to achieving high-purity products and is favored for its high selectivity and controllability. The use of this method to purify GSL is also a method to extract and isolate GSL from natural sources, and it has a wide range of applications in the biopharmaceutical, food, and cosmetic fields. Typical organic solvents include methanol, ethanol, dimethyl sulfoxide, and chloroform, which chemically react with and separate the fatty acids or other impurities in GSLs. In addition, a frequently used subsequent operation is to further purify the product by crystallization and distillation techniques.
Organic solvent purification of sphingolipids is one of the important methods for the biopharmaceutical and food industries to improve the quality of products and the satisfaction of market demanders. CD BioGlyco provides purification services for GSLs of various samples to our customers. Our purification methods include：
Our services effectively separate products from their organic salts or organic impurities by using organic solvents, then distill or remove the solvents to obtain a pure product.
We precipitate the solution with ethanol or methanol and centrifuge it to remove impurities.
We perform chromatography using thin layers of silica gel and organic solvents to separate and purify GSL and remove impurities.
We separate and purify your products by the principle that mixtures and organic solvents move at different speeds through gas chromatography.
Fig.1 The process of GSL purification. (CD BioGlyco)
CD BioGlyco provides an integrated solution of organic solvent-based GSL extraction, we hope our efficient and professional scientific services will help us to be your essential research assistant in the field of glycobiology. If you are interested in our services, please contact us for more detailed information.
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