Overview of PAGE

DNA and RNA are a class of macromolecules with important functions for living organisms, and there is often a requirement for high purity for their use. Therefore, it is a very urgent matter to comprehensively study the way of separating and purifying biomolecules. Among the techniques for the separation and purification of biological macromolecules, gel electrophoresis is a basic technique in the laboratory of biological disciplines. This technique allows efficient separation of macromolecules such as DNA, RNA, and proteins. For proteins in particular, polyacrylamide gel electrophoresis (PAGE) is often the technique of choice. The separation principle of PAGE is based on the electrophoretic mobility of biomolecules (the ability of an analyte to move toward an electrode of opposite charge). In the use of PAGE, the charge, size (molecular weight), and shape of the molecule determine the specific operation.

PAGE-based DNA and RNA Purification Service at CD BioGlyco

For the use of PAGE purification technology, CD BioGlyco has many years of operational experience. The PAGE purification method we offer mainly utilizes denaturing polyacrylamide gel electrophoresis for the separation of primed DNA / RNA, followed by the recovery of the target DNA / RNA from the gel. Currently, the PAGE purification method we offer provides greater than 90% purity of purified DNA and is particularly effective for the purification of long-stranded DNA (greater than 50 mer).

The PAGE purification technique is suitable for the isolation and purification of large polynucleotide DNA or RNA fragments and allows the isolation of relatively small proteins.

• Specifically, CD BioGlyco offers PAGE purification techniques that apply to situations:
  
  • Primers for routine molecular biology experiments
  • Long-chain macromolecules (≥60 mer)
  • Unmodified oligonucleotides over 50 mer
  • Most long strands of DNA or RNA
• Depending on the purpose of the analysis, we offer PAGE including:
  
  • SDS PAGE
  • Native PAGE
• Buffer used
  
  • SDS PAGE uses trimethylene glycol, bis-trimethylene glycol, triacetate, and tris-tri ethylene glycol, of which trimethylene glycol is the most commonly used.
  • Native PAGE uses TBE without SDS.

Fig.1 Flow chart of PAGE purification technology. (CD BioGlyco)

Applications

• PAGE could be used for the purification and analysis of DNA and RNA.
• PAGE could be used for quantitative and structural analysis of proteins.
• PAGE could be used to identify nucleic acid-protein complexes. 

Advantages

• PAGE helps to explore the structure as well as the physiological functions of DNA and RNA.
• PAGE helps to delve into the functions that proteins have, such as cell signaling.

CD BioGlyco has many years of experimental experience in the purification of DNA and RNA. We have professional researchers and first-class experimental equipment to provide custom isolation and purification solutions for clients all over the world. At the same time, we also provide timely feedback to our clients on the progress of their experiments. If you are interested in our services, please feel free to contact us.