Phaseolus lunatus Lectin Production Service

Phaseolus lunatus Lectin Production Service

Phaseolus lunatus Lectin (LBA) Production Service at CD BioGlyco

CD BioGlyco is a trusted provider of Lectin Production services and products. Researchers have isolated and identified several lectins from different P. lunatus species. Because of its wide range of biological functions, LBA has received extensive attention from researchers and has good application prospects. In order to support researchers in all aspects of Plant Lectin Production, and other research, we provide a one-stop LBA production service.

Process of LBA production. (CD BioGlyco) Fig.1 Process of LBA production. (CD BioGlyco)

  • LBA production service

We use P. lunatus seeds and soak them in distilled water for some time. After homogenization, filtration, and centrifugation, we obtain the crude extract of lectin. Afterward, ammonium sulfate is added for precipitation. In order to obtain LBA with high purity, we purify the product obtained after precipitation by Gel Filtration Chromatography, Ion Exchange Chromatography, and Affinity Chromatography in sequence. In order to shorten the test period and obtain LBA with high yield and purity, we optimize and simply modify the experimental solution. The optimized production scheme is tested to improve the purity and yield of LBA.

The molecular weights of lectins, etc., vary among different species of P. lunatus. It is very necessary to test the biochemical properties of LBA obtained from production. We have perfect and advanced testing equipment. Molecular weight, product purity, sugar-binding specificity, amino acid composition, thermal stability, etc., are all tested and analyzed.

  • LBA products

We produce a wide range of labeled LBA conjugate products using purified LBA as the material. Our goal is to meet the different needs of our clients for lectin research.

Publication

Technology: Molecular weight determination

Journal: International Journal of Biological Macromolecules

IF:3.909

Published: 2017

Results: In the present study, P. lunatus seeds were used as experimental material and the crude extract of lectin was obtained by NaCl leaching. Afterward, purified lectin (PLUN) was obtained by filtration through a Sephadex G-100 molecular exclusion column. It was isolated from a protein that only agglutinated human-type A erythrocytes. PLUN was tested to have some antitumor activity and antioxidant capacity.

Chromatogram of purified lectin. Fig.2 Chromatogram of purified lectin. (e Lacerda, et al., 2017)

Applications of LBA Production

  • The LBA we produce is used to analyze cell surface carbohydrate structures and oligosaccharide structures of complex sugars.
  • The LBA we produce is used to study cellular markers of viruses, bacteria, fungi, and other infectious agents.
  • The LBA we produce is used to detect changes in glycan synthesis associated with disease.

Advantages of Us

  • We have a wide range of LBA products with multiple markers to choose from.
  • We are a trusted supplier of lectin products. We obtain high-quality lectin products by optimizing our production methods.
  • We optimize the LBA production method to avoid some of the common problems associated with lectin production, such as lower purity and yield.

CD BioGlyco adheres to the principle of client needs and client satisfaction. We are constantly improving our lectin product quality and production capacity. Our goal is always to provide valuable research products and a higher level of service. If you are interested in our LBA production and various products, please feel free to contact us for detailed information. Our client service staff will give you the most detailed reply the first time.

Reference:

  1. e Lacerda, R.R.; et al. Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L. var. cascavel) presents antioxidant, antitumour and gastroprotective activities. International Journal of Biological Macromolecules. 2017, 95: 1072-1081.
This service is for Research Use Only, not intended for any clinical use.

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