CD BioGlyco is a trusted provider of Lectin Production services and products. Researchers have isolated and identified several lectins from different P. lunatus species. Because of its wide range of biological functions, LBA has received extensive attention from researchers and has good application prospects. In order to support researchers in all aspects of Plant Lectin Production, and other research, we provide a one-stop LBA production service.
Fig.1 Process of LBA production. (CD BioGlyco)
We use P. lunatus seeds and soak them in distilled water for some time. After homogenization, filtration, and centrifugation, we obtain the crude extract of lectin. Afterward, ammonium sulfate is added for precipitation. In order to obtain LBA with high purity, we purify the product obtained after precipitation by Gel Filtration Chromatography, Ion Exchange Chromatography, and Affinity Chromatography in sequence. In order to shorten the test period and obtain LBA with high yield and purity, we optimize and simply modify the experimental solution. The optimized production scheme is tested to improve the purity and yield of LBA.
The molecular weights of lectins, etc., vary among different species of P. lunatus. It is very necessary to test the biochemical properties of LBA obtained from production. We have perfect and advanced testing equipment. Molecular weight, product purity, sugar-binding specificity, amino acid composition, thermal stability, etc., are all tested and analyzed.
We produce a wide range of labeled LBA conjugate products using purified LBA as the material. Our goal is to meet the different needs of our clients for lectin research.
Cat. No. | Product Name | Cat. No. | Product Name |
---|---|---|---|
XLC-55-03Q | LBA, Alkaline Phosphatase Conjugate | XLC-55-05Q-30 | LBA, Colloidal Gold Conjugate, 30 nm |
XLC-55-04Q | LBA, Biotin Conjugate | XLC-55-05Q-40 | LBA, Colloidal Gold Conjugate, 40 nm |
XLC-55-05Q-10 | LBA, Colloidal Gold Conjugate, 10 nm | XLC-55-05Q-5 | LBA, Colloidal Gold Conjugate, 5 nm |
XLC-55-05Q-15 | LBA, Colloidal Gold Conjugate, 15 nm | XLC-55-11Q | LBA, Ferritin Conjugate |
XLC-55-05Q-20 | LBA, Colloidal Gold Conjugate, 20 nm | XLC-55-12Q | LBA, FITC Conjugate |
Technology: Molecular weight determination
Journal: International Journal of Biological Macromolecules
IF:3.909
Published: 2017
Results: In the present study, P. lunatus seeds were used as experimental material and the crude extract of lectin was obtained by NaCl leaching. Afterward, purified lectin (PLUN) was obtained by filtration through a Sephadex G-100 molecular exclusion column. It was isolated from a protein that only agglutinated human-type A erythrocytes. PLUN was tested to have some antitumor activity and antioxidant capacity.
Fig.2 Chromatogram of purified lectin. (e Lacerda, et al., 2017)
CD BioGlyco adheres to the principle of client needs and client satisfaction. We are constantly improving our lectin product quality and production capacity. Our goal is always to provide valuable research products and a higher level of service. If you are interested in our LBA production and various products, please feel free to contact us for detailed information. Our client service staff will give you the most detailed reply the first time.
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