Phaseolus vulgaris Lectin (PHA) Production Service at CD BioGlyco

Plant lectins are all proteins that contain at least one non-catalytic structural domain and bind reversibly to specific monosaccharides or oligosaccharides. It has a wide range of biological activities. CD BioGlyco develops comprehensive Lectin Production to provide clients with Natural Lectin Production Services. Among them, PHA has a large share in plant lectins. At CD BioGlyco, we provide PHA production services to our clients. Moreover, we have prepared many Related Products.

• PHA extraction and purification
• First, we crush and homogenize the Phaseolus vulgaris. Immediately after that saline or buffer is added to it as an extractant to dissolve it. Then the supernatant is collected by ultrasonication and centrifugation operation. Finally, the crude product of PHA is obtained by freezing and drying.
• We purify the crude product further by graded precipitation using ammonium sulfate and Ion Exchange Chromatography techniques. Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry to characterize the protein and determine its purity.
• PHA property assay
• Hemagglutination activity
  The hemagglutination method is utilized to detect the agglutination activity. Buffer is added to the wells of a hemagglutination plate, and different amounts of lectin samples are added to the wells separately, followed by erythrocyte suspension. The coagulant activity of the lectin is observed under the naked eye or microscope.
• Thermal stability
  PHA is dissolved in buffer heat treatment is carried out in a water bath and the hemagglutination activity of the lectin at different temperatures is detected using the hemagglutination method.
• Metal ion binding
  PHA is dissolved in buffer and centrifugation. Different metal ion solutions are added separately and the hemagglutination activity is detected using the hemagglutination method.
• Sugar binding specificity
  We add separate solutions of different sugars to the PHA solution and assay the hemagglutination activity using the hemagglutination assay.

Fig.1 Production content of PHA. (CD BioGlyco)

Publication

Applications

• PHA is highly thermostable, and it is used to develop an efficient yet safe plant-derived biopesticide.
• Based on its ability to bind specifically to sugar groups, PHA is used in a variety of research, such as blood group identification and targeted drug carriers.
• PHA is metal ion-dependent and is used to investigate the principle of food poisoning in kidney beans.

Advantages

• We offer a full range of services from lectin extraction, purification, and property testing, and our clients get comprehensive R&D support from our company.
• Our R&D team consists of a group of senior scientists and technicians with rich experience and strength in the field of lectin production.

CD BioGlyco produces many types of lectins, all of which are highly purified and undergo strict quality control to ensure reproducible research results. At the same time, we offer several different lectin research and development programs to meet the different research needs of our clients. Please feel free to contact us if you would like to learn more about our R&D services.

Reference:

1. Jiang, B.; et al. Two-step isolation, purification, and characterization of lectin from Zihua snap bean (Phaseolus vulgaris) seeds. Polymers. 2019, 11: 785-806.