Phaseolus vulgaris Lectin Production Service

Phaseolus vulgaris Lectin (PHA) Production Service at CD BioGlyco

Plant lectins are all proteins that contain at least one non-catalytic structural domain and bind reversibly to specific monosaccharides or oligosaccharides. It has a wide range of biological activities. CD BioGlyco develops comprehensive Lectin Production to provide clients with Natural Lectin Production Services. Among them, PHA has a large share in plant lectins. At CD BioGlyco, we provide PHA production services to our clients. Moreover, we have prepared many Related Products.

PHA extraction and purification

First, we crush and homogenize the Phaseolus vulgaris. Immediately after that saline or buffer is added to it as an extractant to dissolve it. Then the supernatant is collected by ultrasonication and centrifugation operation. Finally, the crude product of PHA is obtained by freezing and drying.
We purify the crude product further by graded precipitation using ammonium sulfate and Ion Exchange Chromatography techniques. Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry to characterize the protein and determine its purity.

PHA property assay

Hemagglutination activity
The hemagglutination method is utilized to detect the agglutination activity. Buffer is added to the wells of a hemagglutination plate, and different amounts of lectin samples are added to the wells separately, followed by erythrocyte suspension. The coagulant activity of the lectin is observed under the naked eye or microscope.

Thermal stability
PHA is dissolved in buffer heat treatment is carried out in a water bath and the hemagglutination activity of the lectin at different temperatures is detected using the hemagglutination method.

Metal ion binding
PHA is dissolved in buffer and centrifugation. Different metal ion solutions are added separately and the hemagglutination activity is detected using the hemagglutination method.

Sugar binding specificity
We add separate solutions of different sugars to the PHA solution and assay the hemagglutination activity using the hemagglutination assay.

Process of LEL production. Fig.1 Production content of PHA. (CD BioGlyco)

Publication

Technology: Two-step method based on aqueous two-phase system and Sephadex G-75.
Journal: Polymers
IF: 5
Published: 2019
Results: Zihua snap bean was characterized via sodium dodecyl sulfate-polyacrylamide gel electrophoresis, liquid chromatography, mass spectrometry, infrared spectroscopy, and fluorescence spectroscopy for its physicochemical properties and biological activities. The results showed that (I) glucose, N-acetyl-d-glucosamine, galactose, N-acetyl-galactosamine, fructose, sucrose, maltose, alginate, and lactose did not bind well to the lectin. (II) The hemagglutinating activity of the lectin showed a strong dependence on Mn²⁺ and Ca²⁺. (III) Purple-flowering bean curd agglutinin had good thermal and pH stability. (IV) The untreated lectin was relatively stable to pepsin and trypsin activity, but heat treatment significantly reduced digestive stability in vitro. In addition, the lectin was inhibitory to the tested bacteria (S. aureus, E. coli, B. subtilis) and also had an inhibitory effect on bacterial growth.

Fig.2 Inhibition of (a) S. aureus, (b) E. coli, and (c) B. subtilis with different concentrations of lectin. (Jiang, et al., 2019)

Applications

PHA is highly thermostable, and it is used to develop an efficient yet safe plant-derived biopesticide.
Based on its ability to bind specifically to sugar groups, PHA is used in a variety of research, such as blood group identification and targeted drug carriers.
PHA is metal ion-dependent and is used to investigate the principle of food poisoning in kidney beans.

Advantages

We offer a full range of services from lectin extraction, purification, and property testing, and our clients get comprehensive R&D support from our company.
Our R&D team consists of a group of senior scientists and technicians with rich experience and strength in the field of lectin production.

CD BioGlyco produces many types of lectins, all of which are highly purified and undergo strict quality control to ensure reproducible research results. At the same time, we offer several different lectin research and development programs to meet the different research needs of our clients. Please feel free to contact us if you would like to learn more about our R&D services.

Reference:

Jiang, B.; et al. Two-step isolation, purification, and characterization of lectin from Zihua snap bean (Phaseolus vulgaris) seeds. Polymers. 2019, 11: 785-806.

This service is for Research Use Only, not intended for any clinical use.