Properties
Unit Definition
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
Form
buffered aqueous glycerol solution
Purity
buffered aqueous glycerol solution, ≥1, 000 units/mg protein (Lowry)
Applications
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:• in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)• Lyse immunolabeled HeLa cells
