Properties
Description
Phosphotransacetylase from Bacillus subtilis can be used for the interconversion of acetyl phosphate and coenzyme A.
Molecular Weight
36.9 kDa
Unit Definition
One unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25°C.
Applications
Phosphotransacetylase from Bacillus subtilis can be used to determine acetic acid in the food and wine industries.