CD BioGlyco is a world-leading company that can provide the best solutions for global customers in the field of microarrays. We have successfully completed many scientific research and non-clinical research projects to ensure that we provide the best products and services to customers all over the world.

Plant Glycomics Research and Microarray

Most plant polysaccharides, especially those derived from plant cell walls, are complex heteropolymers, and even purified ones contain a series of smaller oligosaccharide substructures (potential epitopes). The heterogeneity and diversity of glycoforms in the polysaccharide category affect the development of this resource and hinders the research on the role of some polysaccharide epitopes in crop physiology and pathology. A detailed understanding of the structure, function, interaction, and occurrence of plant glycans is essential for understanding its complex contribution to plant life and fully utilizing its commercial potential. The microarray and enzyme-linked immunosorbent assay (ELISA) composed of partially defined polysaccharide samples have the characteristics of high-throughput screening.

Fig 1. An illustration of the key steps of Comprehensive Microarray Polymer Profiling (CoMPP). (Moller I E, et al. 2012)

Phyto-glycan Microarray Service

Our services can help research and develop plant cell wall polysaccharide resources, characterize the structure of plant polysaccharides, and develop structural epitopes bound by plant polysaccharides and mAbs that determine the direction of cell wall glycans. Currently, the mainstream microarray-based glycan screening method is Comprehensive Microarray Polymer Profiling (CoMPP), which is very suitable for tracking glycan changes in complex biological systems and providing a global overview of cell wall composition.

• Using a miniaturized microarray platform to screen multiple samples, the spot signal on the microarray can be measured to provide semi-quantitative data about the appearance of glycan epitopes.
• There are currently more than 200 monoclonal antibodies directed against most types of plant cell wall polysaccharides.
• A series of well-defined monoclonal antibodies can be used as molecular probes to infer the accuracy of the presence or abundance of polysaccharides.

Fig 2. Flow chart of CD BioGlyco's plant cell wall glycan array screening service.

The use of synthetic plant carbohydrate microarrays can develop plant cell wall glycan epitope information, help the analysis of polysaccharide structure, and provide the required information for the specific detection of many complex molecular structures in plant cell wall polysaccharides.

CD BioGlyco provides high-quality plant cell wall glycan array screening services. We have a well-equipped experimental platform, a large and professional team of scientists, and we are committed to cooperating with researchers from all over the world to meet the needs of our customers. Customers can contact our employees directly and provide timely feedback on their queries. If you are interested in our one-stop solution service, please contact us for more detailed information.

References:

1. Moller I E, Pettolino F A, Hart C, et al. Glycan profiling of plant cell wall polymers using microarrays[J]. Journal of visualized experiments: JoVE, 2012 (70).
2. Ruprecht C, Bartetzko M P, Senf D, et al. A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies[J]. Plant physiology, 2017, 175(3): 1094-1104.