Properties
Unit Definition
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One unit of PNGase F activity is equal to 1 IUB milliunit.
Purity
BioReagent, ≥95% (SDS-PAGE), for proteomics
Applications
Proteomics Grade PNGase F is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.
