Properties
Unit Definition
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37 °C at pH 7.5 monitored by SDS-PAGE. One unit of PNGase F activity is equal to 1 IUB milliunit.
Form
buffered aqueous solution
Purity
buffered aqueous solution, recombinant, expressed in E. coli
Applications
This product contains low levels of buffer salts. The highly purified material can be used for deglycosylation or for analytical applications in gels, solutions or blotting membranes. It can be removed by using its C-terminal 6x histidine fusion tag. It has been used for the deglycosylation of human plasma samples and chondroitin sulfate proteoglycans.
