Properties
Unit Definition
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One unit of PNGase F activity is equal to 1 IUB milliunit.
Form
buffered aqueous solution
Purity
buffered aqueous solution, recombinant, expressed E. coli
Applications
PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.
