Properties
Description
Porcine lipopolysaccharide (LPS) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of pig LPS as low as 1 ng/mL and its detection range is 25-800 ng/mL.
Assay Principle
Porcine lipopolysaccharide (LPS) ELISA kit is specifically designed for measuring the level of Porcine LPS in a given sample. To perform the assay, purified Porcine LPS antibodies are used to coat the wells of a microtiter plate, creating a solid-phase antibody. Next, the LPS sample is added to the wells, resulting in the binding of LPS molecules to the antibodies. The antibodies are labeled with HRP, forming an antibody-antigen-enzyme-antibody complex. After thorough washing, a TMB substrate solution is added to the wells, causing the TMB substrate to undergo an HRP enzyme-catalyzed reaction, leading to a color change to blue. To terminate the reaction, a sulphuric acid solution is added, and the resulting color change is measured using a spectrophotometer at a wavelength of 450 nm. Finally, the concentration of Porcine LPS in the samples can be determined by comparing the optical density (O.D.) of the samples to a standard curve.
ELISA Type
Sandwich ELISA kit
Assay Type
Sandwich ELISA kit
Components
Closure plate membrane; Sealed bags; Micro ELISA stripplate; Standard; HRP-Conjugate reagent; Sample diluent; Chromogen solution A; Chromogen solution B; Stop solution; Wash solution
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Porcine lipopolysaccharide (LPS) ELISA kit can be used to measure the level of pig LPS in serum, plasma, tissue homogenate and other biological fluid samples.
