Solutions to Prevent Cellular Rejection

Natural killer (NK) cells play a key role in inhibiting tumor growth and metastasis. An increase in sialic acid commonly occurs on the surface of tumor cells. Siglecs are inhibitory receptors that recognize sialic acid ligands, which protect cancer cells from being killed by NK cells. With the support of De Novo Glycan Display, CD BioGlyco provides a range of services from synthesizing sialylated glycan copolymers to analyzing the mechanism of action, advancing the development of our client's projects.

Engineering Cell Surfaces with Synthetic Glycopolymers

• CD BioGlyco provides services to synthesize specific phospholipid-functionalized polymers and implant them on the cell surface. We use synthetic glycopolymers to endow the cell surface with structurally defined glycans to determine the role of sialylated epitopes in cell mediation. Our investigators have rich successful experience in introducing sialidins on the surface of target cancer cells.
• By incubating polymers with multiple cancer cell lines, our technicians provide the abundance and distribution determination services of cell-bound polymers by flow cytometry and fluorescence microscopy. Notably, we optimize the coefficients (e.g., polymer concentration, grant time, polymer structure) to control the density of the polymer.
• The dynamic components of the cell surface will change with the introduction of the glycopolymers. Using biotin-conjugated glycopolymers label the remaining surface-associated polymers with a fluorescent antimicrobial antibody to analyze the cell surface half-life. Our investigators have addressed whether cell surface-bound glycopolymers provide rapid and accurate binding detection services with exogenous receptors.

Sialoside Glycopolymers Protect Mechanism

• Effects of glycocalyx engineering on NK cell-mediated cytotoxicity
• Pathogenic mediator assessment service for sialic acid-based NK cell inhibition
• Analysis of the regulatory effects of the natural ligands
• Effect of sialic acid remodeling in a variety of other tumor cell lines
• Signaling state in the glycopolymer reaction assessment
• In vitro model of porcine aortic epithelial cells
• Coimmunoprecipitation and western blot analysis
• Cytotoxicity analysis

Fig.1 Schematic diagram of preventing cellular rejection analysis service during transplantation. (CD BioGlyco)

Publication

Applications of Preventing Cellular Rejection During Transplantation

• Preventing cellular rejection during transplantation provides a new perspective on immunobiology research. At the same time, it provides a new route for the treatment and research of targeted regulatory receptors.
• Preventing cellular rejection during transplantation opens new avenues to define the role of hyperinflation in cancer immunology.
• Preventing cellular rejection during transplantation is very applicable to the field of NK cells and plays an important role in the study of cancer immunology.

CD BioGlyco has strong background knowledge, superior operators, and experienced staff to help clients quickly reach their demands. If you are interested in our service, please feel free to contact us.