Properties
Description
Pullulanase from Bacillus licheniformis, a glycoside hydrolase, is responsible for catalyzing the cleavage of α-1,6 linkages of pullulan and β-limit dextrin.
Unit Definition
One unit of pullulanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40°C.
Source
Bacillus licheniformis
Applications
Pullulanase from Bacillus licheniformis can be used for research on starch saccharification and the production of high glucose or maltose syrups.