What Do You Know About qRT-PCR?

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is one of the most sensitive genetic analysis techniques available. It has a wide range of applications, including quantitative gene expression analysis, genotyping, drug target validation, biomarker discovery, pathogen detection, and measuring RNA interference. Intensive quality control is a vital part of qRT-PCR that can be captured with plan/prepare, cycle, and report.

RT-qPCR can be done by a one- or two-step method, the difference between which is whether the reverse transcription step and the PCR amplification step are combined.

Fig.1 Schematic diagram of one- or two-step RT-qPCR. (CD BioGlyco)

qRT-PCR-based Glycogene Expression Profiling at CD BioGlyco

As a discovery tool, glycogene expression profiling explains the complex molecular background within organisms and provides excellent insight into new therapies for glycogene-related diseases. CD BioGlyco has comprehensive Platforms to help clients solve various challenges in the field of glycobiology. The workflow of qRT-PCR-based glycogene expression profiling at CD BioGlyco is as follows:

Fig.2 The workflow of qRT-PCR-based glycogene expression profiling. (CD BioGlyco)

With our qRT-PCR-based glycogene expression profiling service, you achieve any goal:

• Study how external factors influence gene expression in cells: qRT-PCR technique compares the relative expression levels of mRNA of specific glycogenes between experimentally treated cells and untreated cells and determines the effects of treatment factors on glycogene expression and cell activities. CD BioGlyco uses sophisticated qRT-PCR techniques to help you get accurate information.
• Study the influence of glycogenes on cell function: By different Strategies for Glycogene Editing, Glycogene Editing combined with qRT-PCR technology clarifies the expression of glycogenes of interest. CD BioGlyco assists clients with our glycogenomics technologies to study the function of specific glycogenes in cells.
• Discover disease-related glycogenes: DNA Microarray and qRT-PCR have been widely used in the search for virulence genes or disease-related genes such as Cancer Glycogenes. CD BioGlyco has advanced equipment and skilled technicians to help clients solve challenges.
• Study mechanism of drug action: With the integrated application of DNA microarray technology and qRT-PCR technology, we assist clients in studying the influence of drugs on the whole gene expression profile and analyze the mechanism of drug action in vivo and in vitro.
• Verify results obtained by other analytical techniques: qTR-PCR has many advantages such as high accuracy, good repeatability, strong specificity, and reliable analytical results. It is often used to verify the accuracy of results obtained by other analytical techniques. We have mastered qRT-PCR technology to help clients obtain reliable glycogene expression profile data.
• Find internal reference genes: qRT-PCR is a powerful tool for understanding metabolic pathways, genetics, signaling pathways, and some complex regulatory networks in organisms. To obtain stable and reliable results in qRT-PCR detection, appropriate and stable internal reference genes are the prerequisite for standardizing gene expression and avoiding errors. We used standardized qRT-PCR operations to help clients search for suitable internal reference genes, ensuring that clients smoothly conduct later research.

Applications

• Study the function of glycogenes in organisms
• Explore and analyze abnormal pathogenic glycogenes
• Discover disease biomarkers
• Construct the diagnosis model of glycogene-related diseases
• Verify data obtained by other analytical techniques

Highlights

• High sensitivity, good repeatability, and high specificity
• Real-time monitoring of response
• Reduce the complexity of sample quantification
• Perform truly quantitative analysis of gene expression

CD BioGlyco provides first-class qRT-PCR-based glycogene expression profiling. We analyze many RNA samples in parallel and offer accurate predictive data for you to ensure the smooth progress of your research. If you are interested in our services, please contact us in time, and we look forward to communicating with you.