Properties
Description
Rat lipopolysaccharide specific IgM antibody (LPS-SIgM) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of rat LPS-SIgM as low as 1.0 ng/mL and its detection range is 12.5-400 ng/mL.
Assay Principle
Rat lipopolysaccharide specific IgM antibody (LPS-SIgM) ELISA kit is intended for laboratory research use only and should not be used for diagnostic or therapeutic procedures. The stop solution included in the kit changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a spectrophotometer. To determine the concentration of LPS-SIgM in the sample, this kit provides a set of calibration standards. These calibration standards are assayed simultaneously with the samples, enabling the operator to generate a standard curve that correlates Optical Density (O.D.) values with LPS-SIgM concentrations. The concentration of LPS-SIgM in the samples can then be determined by comparing their O.D. values to the standard curve.
Components
MicroELISA stripplate; Standard; Sample diluent; Enzyme conjugate; Wash solution; Chromogen solution A; Chromogen solution B; Stop solution; Closure plate membrane; Sealed bags
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
12.5-400 ng/mL
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Cross-reactivity
This assay recognizes recombinant and natural Rat LPS-SIgM. No significant cross-reactivity or interference was observed.
Applications
Rat lipopolysaccharide specific IgM antibody (LPS-SIgM) ELISA kit can be used to measure the level of rat LPS-SIgM in serum, plasma, tissue homogenate and other biological fluid samples.
