Properties
Description
Recombinant endo-β-N-acetylglucosaminidase from Mucor hiemalis is an enzyme that specifically cleaves N-linked glycans from glycoproteins.
Unit Definition
One unit will catalyze the release of 1μmol of pNP-GlcNAc from Disialylnonasaccharide-β-pNP per minute at pH 6.0 at 37°C.
Expression System
Candida boidinii
Production Methods
Fermentation
Sensitivity
Heat sensitive
Concentration
100 munits/vial
Form
White to almost white powder to crystal
Purity
Single band by SDS-PAGE.
Applications
Recombinant endo-β-N-acetylglucosaminidase from Mucor hiemalis is commonly used for glycoprotein analysis, glycan removal, and glycan structure determination.
